Sequence-based engineering of dynamic functions of micrometer-sized DNA droplets

Science Advances
Yusuke SatoMasahiro Takinoue

Abstract

DNA has the potential to achieve a controllable macromolecular structure, such as hydrogels or droplets formed through liquid-liquid phase separation (LLPS), as the design of its base sequence can result in programmable interactions. Here, we constructed "DNA droplets" via LLPS of sequence-designed DNA nanostructures and controlled their dynamic functions by designing their sequences. Specifically, we were able to adjust the temperature required for the formation of DNA droplets by designing the sequences. In addition, the fusion, fission, and formation of Janus-shaped droplets were controlled by sequence design and enzymatic reactions. Furthermore, modifications of proteins with sequence-designed DNAs allowed for their capture into specific droplets. Overall, our results provide a platform for designing and controlling macromolecular droplets via the information encoded in component molecules and pave the way for various applications of sequence-designed DNA such as cell mimics, synthetic membraneless organelles, and artificial molecular systems.

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Citations

Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Omar A SalehTim Liedl
Jan 28, 2021·Nanomaterials·Shu OkumuraAnthony Genot
Jun 13, 2021·Nature Communications·Siddharth AgarwalElisa Franco
Jun 15, 2021·Biophysics and Physicobiology·Yusuke Sato, Yuki Suzuki
Jun 19, 2021·Computational and Structural Biotechnology Journal·Elisa FlorisCarlo C Campa
Aug 10, 2021·Frontiers in Genetics·Marcos Kunihiro MasukawaMasahiro Takinoue
Sep 23, 2020·The Journal of Physical Chemistry. B·Byoung-Jin JeonOmar A Saleh
Sep 10, 2021·Bioconjugate Chemistry·Takuya HidakaSoyoung Park
Nov 10, 2021·Chemical Communications : Chem Comm·Roger Rubio-SánchezLorenzo Di Michele

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Methods Mentioned

BETA
dynamic dissociation
PCR
electrophoresis

Software Mentioned

DINAMelt
NUPACK

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