PMID: 11912186Mar 26, 2002Paper

Sequence dependent instability of mononucleotide microsatellites in cultured mismatch repair proficient and deficient mammalian cells

Human Molecular Genetics
Jayne C BoyerR A Farber

Abstract

We have measured the mutation rates of G(17) and A(17) repeat sequences in cultured mammalian cells with and without mismatch repair and have compared these rates to those of a (CA)(17) repeat sequence. Plasmids containing microsatellites that disrupt the reading frame of a downstream neomycin-resistance gene were introduced into the cells by transfection and revertants were selected using the neomycin analog G418. Comparison of mutation rates within cell lines showed that the mutation rates of A(17) and (CA)(17) sequences were similar in the mismatch repair proficient cells, but the mutation rate of G(17) was significantly higher than that of either A(17) or (CA)(17). In the mismatch repair deficient cells, the G(17) and (CA)(17) mutation rates were similar and were significantly higher than the A(17) rate. PCR analysis of the mutants showed that 1 bp insertions predominated in both mononucleotide repeats in the mismatch repair proficient cells; in mismatch repair deficient cells, 2 bp deletions were the most common mutation in the A(17) sequence, but 1 bp insertions and 2 bp deletions were equally represented in the G(17) sequence. These results indicate that a G(17) repeat is less stable than an A(17) repeat in both mismatch...Continue Reading

Citations

Mar 10, 2010·Journal of Molecular Evolution·Daniel S FalsterJarle Breivik
Dec 17, 2009·Molecular Biotechnology·Atul Bhargava, F F Fuentes
May 22, 2004·Nature Reviews. Genetics·Hans Ellegren
Nov 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jesse J SalkLawrence A Loeb
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Boris P BelotserkovskiiPhilip C Hanawalt
Oct 14, 2011·Genome Research·Yogeshwar D KelkarKateryna D Makova
Nov 23, 2007·Genome Research·Yogeshwar D KelkarKateryna D Makova
Feb 17, 2010·Evolution & Development·Stephen J SalipanteMarshall S Horwitz
Mar 31, 2010·Journal of Clinical Microbiology·Barry G Hall, Stephen J Salipante
Apr 12, 2007·Genome Génome / Conseil National De Recherches Canada·Aïda AzaiezFrançois J Belzile
Feb 5, 2009·BMC Research Notes·James M Thompson, Stephen J Salipante
Sep 2, 2010·Genetics·Tingting GuDacheng Tian
Feb 28, 2013·International Journal of Molecular Sciences·Maria Themeli, Alexandros Spyridonidis
Dec 12, 2012·International Journal of Molecular Sciences·Eirini NeofytouNikolaos M Siafakas
Mar 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephen J Salipante, Marshall S Horwitz
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kamila NaxerovaRakesh K Jain
Oct 19, 2010·Seminars in Cancer Biology·Jesse J Salk, Marshall S Horwitz
Jul 23, 2009·Mutation Research·Claudia P SpampinatoLuciana D Lario
Apr 18, 2009·Genes, Chromosomes & Cancer·Ana M FerreiraRobert M W Hofstra
Sep 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Emmanuel Buschiazzo, Neil J Gemmell
Jan 21, 2015·Nature Reviews. Clinical Oncology·Kamila Naxerova, Rakesh K Jain
Jun 29, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Stephen J Salipante, Barry G Hall
Jul 8, 2017·Science·Kamila NaxerovaRakesh K Jain
Apr 28, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mark D Evans, Marcus S Cooke
Nov 11, 2017·Genome Biology and Evolution·Shujie XiaoWeilong Hao
Aug 21, 2003·Environmental and Molecular Mutagenesis·Nazumi A YamadaRosann A Farber
Oct 17, 2006·American Journal of Medical Genetics. Part a·Weimin BiJames R Lupski
Jul 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Denis A KiktevThomas D Petes
Mar 5, 2020·Genome Research·Christopher Jen-Yue Wei, Kun Zhang
Jun 19, 2007·Cancer Research·Heli SammalkorpiLauri A Aaltonen
Jul 27, 2021·Nucleic Acids Research·Jin H Bae, David Yu Zhang
Aug 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yuezheng ZhangRosa Ana Risques

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.