Sequence determinants for DNA packaging specificity in the S. aureus pathogenicity island SaPI1

Plasmid
Joana C BentoGail E Christie

Abstract

The SaPIs and their relatives are a family of genomic islands that exploit helper phages for high frequency horizontal transfer. One of the mechanisms used by SaPIs to accomplish this molecular piracy is the redirection of the helper phage DNA packaging machinery. SaPIs encode a small terminase subunit that can be substituted for that of the phage. In this study we have determined the initial packaging cleavage sites for helper phage 80α, which uses the phage-encoded small terminase subunit, and for SaPI1, which uses the SaPI-encoded small terminase subunit. We have identified a 19nt SaPI1 sequence that is necessary and sufficient to allow high frequency 80α transduction of a plasmid by a terminase carrying the SaPI1-encoded small subunit. We also show that the hybrid enzyme with the SaPI1 small terminase subunit is capable of generalized transduction.

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Citations

Sep 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Geeta RamRichard P Novick
Jan 9, 2016·Trends in Genetics : TIG·Richard P Novick, Geeta Ram
Dec 17, 2014·Molecular Cell·John ChenRichard P Novick
Mar 10, 2016·Annual Review of Virology·José R Penadés, Gail E Christie
Jun 8, 2019·Microbiology Spectrum·Richard P Novick
Jun 10, 2021·FEMS Microbiology Reviews·Rodrigo Ibarra-ChávezUrvish Trivedi

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