Sequence determinants of GLUT1-mediated accelerated-exchange transport: analysis by homology-scanning mutagenesis.

The Journal of Biological Chemistry
Sabrina S Vollers, Anthony Carruthers


The class 1 equilibrative glucose transporters GLUT1 and GLUT4 are structurally similar but catalyze distinct modes of transport. GLUT1 exhibits trans-acceleration, in which the presence of intracellular sugar stimulates the rate of unidirectional sugar uptake. GLUT4-mediated uptake is unaffected by intracellular sugar. Using homology-scanning mutagenesis in which domains of GLUT1 are substituted with equivalent domains from GLUT4 and vice versa, we show that GLUT1 transmembrane domain 6 is both necessary and sufficient for trans-acceleration. This region is not directly involved in GLUT1 binding of substrate or inhibitors. Rather, transmembrane domain 6 is part of two putative scaffold domains, which coordinate membrane-spanning amphipathic helices that form the sugar translocation pore. We propose that GLUT1 transmembrane domain 6 restrains import when intracellular sugar is absent by slowing transport-associated conformational changes.


Feb 2, 1979·Biochimica Et Biophysica Acta·G F Baker, R J Naftalin
Mar 23, 1979·Biochimica Et Biophysica Acta·Stephen A BaldwinG E Lienhard
Aug 15, 1979·The Biochemical Journal·J D Craik, K R Elliott
Apr 26, 1991·Biochimica Et Biophysica Acta·R J Naftalin, R J Rist
Sep 1, 1990·Journal of Neurochemistry·T M JayL Sokoloff
Oct 17, 1989·Biochemistry·Anthony Carruthers, A L Helgerson
Sep 6, 1985·Science·Mike MuecklerHarvey F Lodish
Dec 10, 1974·Biochimica Et Biophysica Acta·W R Lieb, W D Stein
Dec 10, 1974·Biochimica Et Biophysica Acta·W R Lieb, W D Stein
May 1, 1972·The Biochemical Journal·H S Bachelard
Aug 27, 1966·Nature·O Jardetzky
Nov 11, 1980·Biochemistry·D C Sogin, P C Hinkle
Jul 1, 1981·The Journal of Physiology·P F Baker, A Carruthers
Jan 1, 1995·The American Journal of Physiology·P HansenJ Holloszy
Aug 13, 1996·Biochemistry·Erin K ClohertyAnthony Carruthers
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 8, 2002·American Journal of Physiology. Endocrinology and Metabolism·Hans-Georg JoostBernard Thorens
Oct 16, 2002·Biochemistry·Kara B LevineAnthony Carruthers
Dec 31, 2002·Physiological Reviews·Giovanni E MannLuis Sobrevia
Sep 19, 2006·Biophysical Journal·John HolyoakeMark S P Sansom
Jul 20, 2007·The Journal of General Physiology·David M BlodgettAnthony Carruthers


Jan 26, 2016·Protein Science : a Publication of the Protein Society·Xuejun C Zhang, Lei Han
Dec 7, 2017·The Journal of Membrane Biology·Kenneth P LloydAnthony Carruthers
Nov 22, 2018·The Biochemical Journal·G D Holman
Dec 27, 2016·Biophysics Reports·Xuejun C Zhang, Lei Han
May 31, 2013·The Journal of Biological Chemistry·Julie K De ZutterAnthony Carruthers
Feb 8, 2020·Brain Research·Kelly StarichaSusan Cohen

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.