Sequence determinants of specific pattern-recognition of bacterial ligands by the NAIP-NLRC4 inflammasome

Cell Discovery
Jingyi YangHuimin Yan

Abstract

The NLR apoptosis inhibitory proteins (NAIPs) function as specific cytosolic receptors for bacterial ligands to form the NAIP-NLRC4 inflammasome for anti-bacterial defenses. In mice, NAIP5/6 and NAIP2 recognize bacteria flagellin and the rod protein of the type III secretion system (T3SS), respectively. However, molecular mechanism for specific ligand pattern-recognition by the NAIPs is largely unknown. Here, through extensive domain swapping and truncation analyses, three structural domains, the pre-BIR, BIR1, and HD1, in NAIP2 and NAIP5 are identified, that are important for specific recognition of their respective ligand(s). The three domains are sufficient to confer the ligand specificity for NAIP2. Asp-18, Arg-108, and Arg-667, respectively, in the pre-BIR, BIR1 and HD1 of NAIP2 are further identified, each of which is essential for efficient binding to the rod protein. To our surprise, we find that the C-terminal leucine-rich repeat domain is dispensable for NAIP2 recognition of the T3SS rod protein, but is required for NAIP5 binding to flagellin. At the ligand side, we discover that the C-terminal 35 residues in flagellin are crucial for binding to NAIP5. Among the 35 residues, three critical residues are identified, whi...Continue Reading

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Citations

Feb 26, 2019·Microbial Biotechnology·Grégory BoëlAntoine Danchin
Sep 14, 2018·Microbiology and Molecular Biology Reviews : MMBR·Jenni A HaywardSi Ming Man
Jan 1, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna M GramClare E Bryant

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Methods Mentioned

BETA
two-hybrid
electrophoresis
co-immunoprecipitation
co-immunoprecipitation assay
transfection
targeted mutation
immunoprecipitation
ELISA

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis