Sequence determination of alphas1-casein isoforms from donkey by mass spectrometric methods

Journal of Mass Spectrometry : JMS
Vincenzo CunsoloSalvatore Foti

Abstract

Four co-eluting components, with experimentally measured M(r) of 23 658, 23 786, 24 278 and 24 406 Da, were detected by reversed-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis in the dephosphorylated casein fraction of a milk sample collected at middle lactation stage from an individual donkey belonging to the Ragusano breed. By coupling RP-HPLC, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), enzymatic digestions, MALDI-TOF MS and capillary RP-HPLC/nano-electrospray ionization tandem mass spectrometry (nESI-MS/MS) analyses, the four components were identified as donkey's alpha(s1)-CNs and their sequences completely characterized, using the known mare's alpha(s1)-CN (GenBank Acc. No. AAK83668; M(r) 23750.7 Da) as reference. The proteins with M(r) of 23 786 and 23 658 Da differ in the presence of a glutamine residue at position 83 in the full-length component and present the amino acid substitutions Q(8)-->H and H(115)-->Y with respect to the mare's alpha(s1)-CN. The other two components with M(r) 24 406 and 24 278 Da, which also differ in the presence of a glutamine residue at position 88 in the full-le...Continue Reading

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Jul 3, 2007·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
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May 23, 2009·Rapid Communications in Mass Spectrometry : RCM·Vincenzo CunsoloSalvatore Foti

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Citations

Jun 8, 2011·Journal of Proteomics·Vincenzo CunsoloSalvatore Foti
May 30, 2012·Journal of Proteomics·Paola RoncadaLuigi Bonizzi
May 1, 2019·The Journal of Dairy Research·Gianfranco CosenzaAlfredo Pauciullo
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Rosaria SalettiSalvatore Foti
Sep 18, 2014·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti

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