Sequence divergence and conservation in genomes of Helicobacter cetorum strains from a dolphin and a whale

PloS One
Dangeruta KersulyteDouglas E Berg

Abstract

Strains of Helicobacter cetorum have been cultured from several marine mammals and have been found to be closely related in 16 S rDNA sequence to the human gastric pathogen H. pylori, but their genomes were not characterized further. The genomes of H. cetorum strains from a dolphin and a whale were sequenced completely using 454 technology and PCR and capillary sequencing. These genomes are 1.8 and 1.95 mb in size, some 7-26% larger than H. pylori genomes, and differ markedly from one another in gene content, and sequences and arrangements of shared genes. However, each strain is more related overall to H. pylori and its descendant H. acinonychis than to other known species. These H. cetorum strains lack cag pathogenicity islands, but contain novel alleles of the virulence-associated vacuolating cytotoxin (vacA) gene. Of particular note are (i) an extra triplet of vacA genes with ≤50% protein-level identity to each other in the 5' two-thirds of the gene needed for host factor interaction; (ii) divergent sets of outer membrane protein genes; (iii) several metabolic genes distinct from those of H. pylori; (iv) genes for an iron-cofactored urease related to those of Helicobacter species from terrestrial carnivores, in addition to ...Continue Reading

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Sep 22, 2015·Scientific Reports·F F ValeP Lehours
Aug 30, 2014·Helicobacter·Armelle MénardBram Flahou
Jun 9, 2016·Toxins·Nora J FoegedingTimothy L Cover
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Jun 24, 2018·International Journal of Molecular Sciences·Filipa F Vale, Philippe Lehours
Jan 17, 2019·PeerJ·Jerson Alexander García-ZeaCarmelo Ruiz Rejón

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Datasets Mentioned

BETA
CP003481.1
CP003482.1
PRJNA178201

Methods Mentioned

BETA
PCR
DNA exchange

Software Mentioned

jEMBOSS
DISTMAT
BLASTP
perl
BLAST
BLASTN
OrthoMCL
Mega BLAST
- FFT - NS - i
Translatorx perl script

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