PMID: 7017718Jan 1, 1981Paper

Sequence homologies between A subunits of Escherichia coli and Vibrio cholerae enterotoxins

Proceedings of the National Academy of Sciences of the United States of America
E K SpicerD E Schafer

Abstract

The genes coding for the heat-labile enterotoxin LT produced by Escherichia coli have been cloned into the plasmid pBR313. Using DNA derived from the resulting chimeric plasmid, we determined the nucleotide sequence of two regions of the gene coding for the enzymatically active A subunit of LT. Translation of the nucleotide sequence gives the primary structure of the NH2-terminal and COOH-terminal regions of the LT A subunit. This permits direct comparison of the LT A subunit with the A subunit of cholera toxin. Our results show that the two toxins possess homologous sequences, of varying degrees, in both regions of their primary structure. The order of the component A1 and A2 polypeptides is A1-A2. The nucleotide sequence predicts the existence of a signal sequence of 18 amino acids at the NH2-terminus of the A subunit.

References

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Citations

Jan 1, 1982·The Journal of Membrane Biology·P H Fishman
Jan 1, 1985·Pharmacology & Therapeutics·D C RobertsonF Dorner
Jan 1, 1985·Pharmacology & Therapeutics·R A Finkelstein, F Dorner
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G D Pearson, J J Mekalanos
Aug 25, 1982·Nucleic Acids Research·M L GennaroD A Broadbent
Jun 25, 1983·Nucleic Acids Research·M L Gennaro, P J Greenaway
Jul 11, 1984·Nucleic Acids Research·M E Watson
Aug 15, 1982·Journal of Molecular Biology·G von Heijne
Nov 13, 2007·FEMS Microbiology Letters·J Daniel Dubreuil
Jun 19, 2004·Molecular Membrane Biology·Lolke De Haan, Timothy R Hirst
Sep 23, 1985·FEBS Letters·D M Burns, I R Beacham
Oct 1, 1994·Molecular Microbiology·M DomenighiniR Rappuoli
Mar 1, 1982·Bioscience Reports·S van Heyningen
Jun 1, 1982·Journal of Bacteriology·T Yamamoto, T Yokota
Dec 1, 1983·Microbiological Reviews·L EidelsD A Hart
Jul 1, 1982·Journal of Clinical Microbiology·J B KaperS Falkow
Sep 1, 1981·Infection and Immunity·D M GillR A Finkelstein

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