Sequence information, distinction and quantitation of C-terminal leucine and isoleucine in ternary complexes of tripeptides with Cu(II) and 2,2'-bipyridine

Journal of Mass Spectrometry : JMS
Tomás VaisarFrantisek Turecek

Abstract

Tripeptides form ternary complexes with Cu(2+) and 2,2'-bipyridine (bpy) that self-assemble upon mixing the components in aqueous methanol solution. Electrospray ionization (ESI) of the complex solutions provides abundant singly charged [Cu(peptide -- H)bpy](+) and doubly charged [Cu(peptide)bpy](2+) ions. Collision-induced dissociation (CID) at low ion kinetic energies of several tripeptides, AGG, GGA, LGG, GGL, GGI, FGG, GGF, LGF, GLF, GFL, GYA and GAY, showed fragments that were indicative of the amino acid sequence in the peptide. In addition, CID of single and doubly charged complexes of isomeric tripeptides GGL and GGI provided unambiguous distinction of the isomeric leucine and isoleucine residues. Leucine peptides eliminated C(3)H(7) radicals from the amino acid side-chain whereas isoleucine eliminated C(2)H(5) radicals. CID of gas-phase doubly charged peptide complexes in a quadrupole ion trap produced a series of singly charged sequence fragments that following isolation and further CID furnished distinct fragments that allowed quantitation of leucine and isoleucine-containing peptides in mixtures.

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Citations

Mar 5, 2004·Journal of the American Society for Mass Spectrometry·Alexandre A Shvartsburg, Richard C Jones
Jul 6, 2004·Journal of the American Society for Mass Spectrometry·Sheldon M Williams, Jennifer S Brodbelt
Sep 1, 2004·Journal of the American Society for Mass Spectrometry·Barry D Davis, Jennifer S Brodbelt
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Erik A Syrstad, Frantisek Turecek
Oct 4, 2005·Journal of the American Society for Mass Spectrometry·Ivan K Chu, Corey N W Lam
Mar 6, 2007·Journal of the American Society for Mass Spectrometry·Chagit Denekamp, Emilia Rabkin
May 9, 2013·Chemical Reviews·František Tureček, Ryan R Julian
Jan 24, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Liyun ZhangLifang Wu
May 28, 2014·Mass Spectrometry Reviews·Han Bin Oh, Bongjin Moon
Jul 1, 2006·Journal of Mass Spectrometry : JMS·Barry D DavisJennifer S Brodbelt
Aug 5, 2006·Journal of Mass Spectrometry : JMS·Mélanie LagarrigueJean-Claude Tabet
May 3, 2007·Mass Spectrometry Reviews·Frantisek Turecek
Dec 7, 2006·Journal of Mass Spectrometry : JMS·Michel BoutinJean-Claude Tabet
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Apr 17, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·M Hassan KhodabandehGilles Ohanessian
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