Sequence of hepatitis G virus genome isolated from a Japanese patient with non-A-E-hepatitis: amplification and cloning by long reverse transcription-PCR

Biochemical and Biophysical Research Communications
L ShaoT Takahashi

Abstract

The nucleotide sequence of hepatitis G virus (HGV) genome was determined by analysis of cDNA clones obtained by long reverse transcription-polymerase chain reaction (long RT PCR) and 5'- and 3'-rapid amplification of cDNA ends (RACE) from a Japanese patient (Iw) with non A-E hepatitis. The HGV-Iw genome, consisting of 9375 nucleotides, contains a long open reading frame encoding 2873 amino acid residues. Comparison of HGV-Iw with two American isolates of HGV and one African isolate of GB virus C (GBV-C) indicates that although the nucleotide sequences of these isolates were considerably divergent (86.2% to 93.3%), the deduced amino acid sequences shared an extremely high degree of identity (96.1% to 100%). It was also found that HGV-Iw was more closely related to the HGV isolates from USA than to the GBV-C isolate from Africa.

Citations

Aug 13, 1998·Journal of Medical Virology·E LampeC Niel
Jan 4, 1998·Journal of Medical Virology·H M HassobaT L Wright
Mar 3, 2005·World Journal of Gastroenterology : WJG·Hao RenZhong-Tian Qi
Aug 26, 1998·Liver·M J MphahleleW F Carman
Feb 17, 2000·The Journal of General Virology·D B SmithP Simmonds
Mar 2, 1999·Biochemical and Biophysical Research Communications·R N CharrelX de Lamballerie
Sep 1, 1997·Journal of Virology·Y E KhudyakovH A Fields
Mar 13, 1999·Journal of Clinical Microbiology·H NaitoK Abe

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