PMID: 9182854Jan 1, 1997Paper

Sequence of mouse 17beta-hydroxysteroid dehydrogenase type 3 cDNA and tissue distribution of the type 1 and type 3 isoform mRNAs

The Journal of Steroid Biochemistry and Molecular Biology
J A ShaP J O'Shaughnessy

Abstract

The enzyme 17beta-hydroxysteroid dehydrogenase (17betaHSD) interconverts 17-ketosteroids and 17beta-hydroxysteroids. Five isoforms of the enzyme have been identified in the human, two of which (types 1 and 3) have been shown to catalyse the reduction reaction preferentially. The cDNA encoding mouse 17betaHSD type 3 was isolated from testis cDNA using the RACE technique and primers based on the human sequence. The mouse protein is 305 amino acids in length which is five short of the human protein with four of these amino acids missing at the N-terminus. The predicted amino acid sequence is 72.5% identical and 94.8% similar to the human sequence. Tissue distribution of mRNA encoding both types 1 and 3 17betaHSD was studied using reverse transcription and the polymerase chain reaction (RT-PCR). Highest levels of type 1 mRNA were found in the ovary whereas highest levels of type 3 were in the testis. All other tissues tested contained mRNA encoding both isoforms of the enzyme although levels were markedly lower than in the gonads.

References

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Citations

May 8, 2004·Molecular and Cellular Endocrinology·R MindnichJ Adamski
Mar 12, 2003·Steroids·Nirmal S PanesarChung S Ho
Feb 13, 2001·Molecular and Cellular Endocrinology·V Luu-TheF Labrie
May 29, 2003·Molecular and Cellular Endocrinology·Mamoru TanakaEli Y Adashi
Dec 24, 1997·Molecular and Cellular Endocrinology·P J BakerP J O'Shaughnessy
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·M L DufauA Khanum
Apr 20, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Nobuyuki SakuraiHiroshi Handa
Jan 3, 2006·Bioorganic & Medicinal Chemistry Letters·Brian E FinkGregory D Vite
Sep 29, 1999·Biochimica Et Biophysica Acta·P RheaultV Luu-The
Jun 13, 2006·Biochimica Et Biophysica Acta·Steven HuyghePaul P Van Veldhoven
Aug 8, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Shuji OhnoShizuo Nakajin
Dec 30, 2008·Molecular and Cellular Endocrinology·R Mindnich, J Adamski
Jan 5, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Sandrine Marchais-OberwinklerJerzy Adamski

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