Sequence requirements for Rev multimerization in vivo

Virology
S J MadoreB R Cullen

Abstract

Multimerization of the human immunodeficiency virus type 1 (HIV-1) Rev protein is believed to be critical to its biological activity. However, the precise protein sequence requirements for Rev multimerization in vivo, and whether multimerization is facilitated by specific RNA binding or vice versa, has remained controversial. In this report, we describe a sensitive in vivo assay for the multimerization of HIV-1 Rev on its cognate RRE primary RNA binding site. Using this assay, we demonstrate that an intact Rev arginine-rich domain, while critical to specific RNA binding, is dispensable for multimerization on the RRE. Mutations introduced into Rev sequences that flank this basic domain produce a partial multimerization phenotype in vivo even though these mutations are known to block Rev multimerization in vitro. Similarly, mutations introduced into the leucine-rich activation domain of Rev, which appear to have no effect on in vitro multimerization, also markedly inhibit multimerization of Rev on the RRE in vivo. Overall, these data appear consistent with the hypothesis that in vivo formation of the multimeric Rev:RRE ribonucleoprotein complex is facilitated by both the RRE RNA substrate and, as first proposed by Bogerd and Gree...Continue Reading

Citations

Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·X Y QianR M Krug
Jul 28, 2009·The Journal of Biological Chemistry·Jennifer A MertzJaquelin P Dudley
Aug 9, 2002·AIDS Research and Human Retroviruses·Jianhua FangRoger J Pomerantz
Dec 29, 2005·AIDS Research and Human Retroviruses·Rajesh RamakrishnanNafees Ahmad
Oct 7, 2008·Nucleic Acids Research·Matthias MüllnerStanislav Indik
Jan 19, 1999·Annual Review of Microbiology·V W Pollard, M H Malim
Jun 14, 2005·Retrovirology·Clemens FurnesAnne Marie Szilvay
Mar 2, 2005·Virology Journal·Liliana Endo-MunozNigel A J McMillan
Sep 24, 2011·Viruses·Myles H Hammarskjold, David Rekosh
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·O SchatzJ Hauber
Mar 14, 1997·Journal of Molecular Biology·B CharpentierM Rosbash
Nov 30, 2004·Virology·Jianhua FangRoger J Pomerantz
Jul 12, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Shreesh SauryaAbraham Karpas
Jan 1, 2012·Biology·Anna Kula, Alessandro Marcello
Mar 25, 2015·Genes·Masumi OkamuraSeiji Masuda
Aug 6, 2004·The Journal of Biological Chemistry·Dirk DaelemansGeorge N Pavlakis
Oct 9, 2002·Medicinal Research Reviews·Elena A LesnikDavid J Ecker
May 18, 2006·The Journal of Biological Chemistry·Marc ArnoldRalph H Kehlenbach
May 10, 2011·Journal of Animal Science·V Rivera-TorresD Sauvant

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