PMID: 3754635Apr 25, 1986Paper

Sequence signals which may be required for efficient formation of mRNA 3' termini

Nucleic Acids Research
R Nussinov

Abstract

It has been known for sometime that AATAAA (Proudfoot and Brownlee, 1976) is often required for 3' mRNA processing. More recently, McLauchlan et al. (1985) have shown the high incidence of YGTGTTYY (Y is a pyrimidine) downstream from the poly (A) addition site. Our results fully support their findings. There have been indications that additional sequences are required either for transcription termination of mRNA or for its cleavage/processing. Here I present the results of detailed analysis of mammalian DNA sequences around the 3' ends of mRNAs. The distributions of 256 quartets and some pentamers have been studied. Except for the AATAAA several additional signals emerge, namely, the homooligomers A4-5, T4-5 and C4-5, C3-4 interspersed with a single T, alternations of T and G, TTCTT and GGAGG. These are highly regular sequences which may exhibit unique conformations. The results also show a clear-cut asymmetry in the distribution of complementary oligomers on the same DNA strand. The possibility that at least some of these signals are recognized on the DNA and thus play a role in the termination process is discussed.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Levitt
Sep 16, 1976·Nature·N J Proudfoot, G G Brownlee
Oct 1, 1969·Journal of Cellular Physiology·R Langridge
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Salditt-Georgieff, J E Darnell
Jul 15, 1981·Journal of Molecular Biology·G P LomonossoffA Klug
Jun 1, 1984·Cell·H R Drew, A A Travers
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S CheungP Lu
May 25, 1984·Science·B de CrombruggheH Buc
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·P LuK Arndt
Jan 1, 1984·Bulletin of Mathematical Biology·M S WatermanD J Galas
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·S ArnottM Wang
May 25, 1984·Journal of Molecular Biology·G G Lennon, R Nussinov

❮ Previous
Next ❯

Citations

Sep 1, 1988·Plant Molecular Biology·J M Martinez-ZapaterC R Somerville
Jan 1, 1988·The International Journal of Biochemistry·R Nussinov
Feb 5, 2000·Genome Génome / Conseil National De Recherches Canada·E N YoshidaD A Hickey
Jul 8, 1988·Journal of Theoretical Biology·R Nussinov
Jul 1, 1987·Archives of Biochemistry and Biophysics·P L IversenR N Hines
Jun 1, 1987·Journal of Biomolecular Structure & Dynamics·R Nussinov
Mar 21, 1987·Journal of Theoretical Biology·R Nussinov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.