Sequence-specific binding of androgen-receptor complexes to prostatic binding protein genes

Molecular and Cellular Endocrinology
F ClaessensW Rombauts

Abstract

Prostatic binding protein is a complex glycoprotein comprising three components, C1, C2 and C3, organized into two different heterodimers (C1-C3 and C2-C3). The rat ventral prostate genes encoding all three constituent polypeptides are expressed under androgenic control. Analysis of genomic fragments containing the genes and flanking sequences revealed in each case one androgen receptor-binding region upstream of or within the promoter and another in the first intron. The effect of androgens on the expression of these genes may, therefore, be mediated by these direct receptor-DNA interactions. The genomic fragments which contain androgen receptor-binding regions all contain nucleotide sequences reminiscent of glucocorticoid response elements (GRE). Mutations in these sequences in restriction fragments and in synthetic oligonucleotides significantly decreased their affinity for androgen-receptor complexes and their introduction into nonspecific sequences conferred affinity for androgen-receptor complexes. Based on these data, a consensus sequence for putative androgen response elements (ARE) is proposed. However, despite the specific recognition of these sequences by the androgen receptor in vitro, only the C3(1) intronic fragme...Continue Reading

References

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Citations

Apr 1, 1991·Molecular and Cellular Endocrinology·S J Higgins, A L Hemingway
Oct 1, 1992·Molecular and Cellular Endocrinology·L J BlokJ A Grootegoed
Aug 1, 1993·Molecular and Cellular Endocrinology·E R GauthierJ Y Dubé
Aug 1, 1993·Molecular and Cellular Endocrinology·L CelisW Rombauts
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·G VerhoevenW Heyns
Jul 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·E PailhouxC Jean
Dec 24, 1997·Molecular and Cellular Endocrinology·S A Nelson, D M Robins
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·F ClaessensW Rombauts
Jun 11, 1993·Annals of the New York Academy of Sciences·F ClaessensW Rombauts
Dec 13, 2005·Andrologia·F ClaessensN Van Tilborgh
Jan 23, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Rebecca Lee Yean WongCheryl Lyn Walker
Jul 10, 2008·Nuclear Receptor Signaling·Frank ClaessensAnnemie Haelens
Oct 1, 1995·Biological Chemistry Hoppe-Seyler·K FukuchiK Gomi

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