PMID: 2120703Oct 1, 1990Paper

Sequence-specific DNA binding by glucocorticoid receptor "zinc finger peptides"

Proceedings of the National Academy of Sciences of the United States of America
T K ArcherJ G Omichinski

Abstract

Steroid hormone receptors can activate or repress transcription from responsive loci by binding to DNA. We have examined the mechanism of DNA binding by individually synthesizing the putative "zinc finger peptides" from the rat glucocorticoid receptor. Atomic absorption studies show that the peptides will bind zinc on an equimolar basis, and circular dichroism experiments demonstrate a significant alteration in secondary structure in the presence of zinc. The results from a series of experiments establish that metal ion is required for binding to DNA and that the amino-terminal zinc finger shows a significantly greater affinity for glucocorticoid response element-containing DNA over control DNA. These observations indicate that a single synthetic "zinc finger peptide" is able to bind to DNA in a sequence-specific manner.

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Citations

Aug 1, 1995·Molecular Biotechnology·D C HancockG I Evan
Oct 1, 1997·Science in China. Series C, Life Sciences·R WangX Pan
Mar 20, 2004·Pharmacology & Therapeutics·Janine L Steiger, Shelley J Russek
Oct 4, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Bonnie J Deroo, Trevor K Archer
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Y Louie, T J Meade
Nov 6, 2001·Molecular Biology of the Cell·B J Deroo, T K Archer
Oct 22, 2008·The International Journal of Biochemistry & Cell Biology·Anuja A GeorgeGordon L Hager
Jul 30, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Leila Maurmann, Rathindra N Bose
Nov 5, 1997·The Journal of Biological Chemistry·H ChenE B Thompson
May 5, 2004·The Journal of Biological Chemistry·S Anuradha, K Muniyappa
Jan 1, 1992·Acta Obstetricia Et Gynecologica Scandinavica. Supplement·J A HoltM W Beckmann
Sep 6, 2001·Oncogene·D N DiNardoD I Rodenhiser
Jan 10, 1997·The Journal of Biological Chemistry·B A Lieberman, S K Nordeen
May 18, 2012·Journal of the American Chemical Society·Anne M RichDean E Wilcox

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