Sequence-specific enhancer binding protein is responsible for the differential expression of ERT/ESX/ELF-3/ESE-1/jen gene in human gastric cancer cell lines: Implication for the loss of TGF-beta type II receptor expression

Oncogene
S H ParkS J Kim

Abstract

Transcriptional repression of the TGF-beta type II receptor (RII) is one of the mechanisms leading to TGF-beta resistance. The newly identified epithelium-specific ets transcription factor ERT/ESX/ELF-3/ESE-1/jen binds to the TGF-beta RII promoter and induces promoter activity. The human gastric cancer cell lines, which show undetectable level of TGF-beta RII mRNA, do not express ERT mRNA. To study the molecular mechanisms of loss of ERT expression, we have cloned and characterized the human ERT promoter. DNA transfection experiments and electrophoretic mobility shift assays have revealed the existence of a distinct enhancer element (-186 to -177) which we named ESE (ERT promoter specific element). Deletion of the ESE markedly decreased expression of the target gene. ESE interacts with two distinct nuclear protein complexes, at least one of which appears to be inactivated in a cell line which does not express the ERT mRNA, compared to a cell line expressing the ERT mRNA. These results suggest the possibility that inactivation of the sequence-specific DNA binding protein to the region from -186 to -177 contributes to the loss of ERT expression, leading to the loss of TGF-beta type II receptor mRNA in human gastric cancer cell li...Continue Reading

References

Jul 1, 1992·Trends in Biochemical Sciences·K MacleodD Stehelin
May 1, 1992·Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research·A SethT S Papas
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C MisseroG P Dotto
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M E KadinD George
Aug 4, 1994·Nature·J L WranaJ Massagué
Jun 1, 1996·Cytokine & Growth Factor Reviews·S D Markowitz, A B Roberts
Oct 27, 1997·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·M Z Zdzienicka
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Nov 7, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R NeveC C Benz
May 1, 1994·Trends in Cell Biology·J MassaguéJ L Wrana

❮ Previous
Next ❯

Citations

Jul 16, 2003·Cancer Letters·Hyang Ran JuInpyo Choi
Jul 29, 2008·Connective Tissue Research·Jean-Pierre PujolKarim Boumediene
Oct 27, 2015·Cancer Letters·Kyung-Won MinSeung Joon Baek
Nov 26, 2005·Gene·Richard M NeveChristopher C Benz
Dec 14, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jordan R OliverJim Hu
Oct 12, 2005·The Journal of Urology·Shahrokh F ShariatYair Lotan
Oct 9, 2003·The Journal of Urology·Dah-Shyong YuSun-Yran Chang
Sep 18, 2007·The Journal of Urology·Xu HuangAria F Olumi
Dec 23, 2015·PloS One·Catherine WestbomArti Shukla
Jul 28, 2016·Science China. Life Sciences·Jiawei GuoXiufeng Pang
Apr 6, 2007·Gastroenterology·Nicole FlentjarMelanie A Pritchard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.