Sequence-specific hypomethylation of the tobacco genome induced with dihydroxypropyladenine, ethionine and 5-azacytidine

FEBS Letters
A KovaříkM Bezděk

Abstract

Higher plant DNA is methylated at CG and CNG targets. In this study we have investigated the tobacco methylation system in tissue culture using the methylation inhibitors 5-azacytidine (5-azaC), dihydroxypropyladenine (DHPA) and ethionine (Ethi), and methylation-sensitive restriction endonucleases HpaII, MspI, HhaI, EcoRII, ScrFI, and Fnu4HI. Surprisingly, CAG/CTG sequences, contrary to CG doublets and CCG/CGG triplets, appeared to be refractory to the inhibitory effect of 5-azaC. Thus 5-azaC cannot be considered a general inhibitor of DNA methylation in tobacco cells. On the other hand, DHPA, the inhibitor of S-adenosylhomocysteine (SAH) hydrolase, and Ethi caused hypomethylation of both CAG/CTG and CCG/CGG triplets but not of the CG doublets. The sensitivity of triplet-specific methylation to the inhibition of SAH hydrolase suggests the possibility that plant-specific DNA methylation at CNG targets might be modulated by alterations of the SAH/S-Adenosylmethionine ratio in plant cells.

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Citations

Sep 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B VyskotM Bezděk
Aug 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·R MatyásekM Bezdĕk
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·E. J. FinneganE. S. Dennis
Mar 18, 2011·Current Opinion in Plant Biology·Marc De Block, Mieke Van Lijsebettens
Jul 11, 2013·Plant Signaling & Behavior·Huiming ZhangJian-Kang Zhu
May 8, 2010·Plant Signaling & Behavior·Kiyotoshi Takeno
Mar 23, 2020·Molecular Metabolism·Christian LindermayrMartin Groth
Aug 23, 2021·Molecular Biology Reports·Fatemeh RavanrouySeyed Mohsen Taghavi

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