PMID: 11345436May 10, 2001Paper

Sequence-specific interaction between HIV-1 matrix protein and viral genomic RNA revealed by in vitro genetic selection

RNA
P PurohitM R Green

Abstract

The human immunodeficiency virus type-1 matrix protein (HIV-1 MA) is a multifunctional structural protein synthesized as part of the Pr55 gag polyprotein. We have used in vitro genetic selection to identify an RNA consensus sequence that specifically interacts with MA (Kd = 5 x 10(-7) M). This 13-nt MA binding consensus sequence bears a high degree of homology (77%) to a region (nt 1433-1446) within the POL open reading frame of the HIV-1 genome (consensus sequence from 38 HIV-1 strains). Chemical interference experiments identified the nucleotides within the MA binding consensus sequence involved in direct contact with MA. We further demonstrate that this RNA-protein interaction is mediated through a stretch of basic amino acids within MA. Mutations that disrupt the interaction between MA and its RNA binding site within the HIV-1 genome resulted in a measurable decrease in viral replication.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C TuerkL Gold
Oct 1, 1992·AIDS Research and Human Retroviruses·A G Bukrinskaya Tentsov YYu
Apr 1, 1989·Genes & Development·B BowermanH E Varmus
Dec 8, 1995·Journal of Molecular Biology·J L DarlixB P Roques
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M I BukrinskyM Stevenson
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R WyattD J Ecker
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·U von SchwedlerD Trono
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·N K HeinzingerM Emerman
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K SakaguchiE Appella
Feb 20, 1996·Biochemistry·J Tao, A D Frankel
Mar 1, 1997·Nucleic Acids Research·J A BerglundM Rosbash

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Citations

Sep 14, 2007·Archives of Virology·S C B Gopinath
Oct 29, 2013·Protein Expression and Purification·Dominic F Qualley, Bethany L Boleratz
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Vineela ChukkapalliAkira Ono
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cristina GhergheKevin M Weeks
Nov 9, 2012·The Journal of Biological Chemistry·Ayna AlfadhliEric Barklis
Apr 7, 2007·AIDS Research and Human Retroviruses·Anna C Hearps, David A Jans
Dec 3, 2010·Journal of Virology·Christopher P JonesKarin Musier-Forsyth
Oct 17, 2006·Journal of Virology·Emma C Anderson, Andrew M L Lever
Sep 16, 2011·Journal of Virology·Siddhartha A K DattaAlan Rein
Sep 21, 2012·Journal of Virology·Ina P O'CarrollAlan Rein
Oct 28, 2004·Journal of Virology·Delphine MuriauxAlan Rein
Mar 8, 2012·Molekuliarnaia biologiia·G K VorkunovaA G Bukrinskaia
Aug 16, 2011·Physiology·Muthukumar Balasubramaniam, Eric O Freed
Mar 10, 2011·Retrovirology·Elise Hamard-Peron, Delphine Muriaux
Jan 1, 2014·Uirusu·Akira Ono
Jul 19, 2011·Journal of Molecular Biology·Vineela Chukkapalli, Akira Ono
Jul 19, 2011·Journal of Molecular Biology·Kun LuMichael F Summers
Jul 19, 2011·Journal of Molecular Biology·Leslie J Parent, Nicole Gudleski
Jul 19, 2011·Journal of Molecular Biology·Ayna AlfadhliEric Barklis
Dec 8, 2010·Journal of Molecular Biology·Siddhartha A K DattaHirsh Nanda
Mar 7, 2009·Analytica Chimica Acta·Subash Chandra Bose Gopinath
Mar 19, 2016·Retrovirology·Katarzyna Pachulska-WieczorekKatarzyna J Purzycka
May 20, 2015·Journal of Molecular Biology·Marc PotempaRonald Swanstrom
Mar 10, 2015·AIDS Research and Human Retroviruses·Chandra Nath RoyHitoshi Oshitani
Mar 17, 2009·Virology·Jun DouPaul Spearman
Jul 8, 2014·Virus Research·Balaji Olety, Akira Ono
Jun 12, 2014·Frontiers in Microbiology·Ayna Alfadhli, Eric Barklis
Jul 11, 2014·Frontiers in Microbiology·José O MaldonadoLouis M Mansky
Oct 17, 2014·Journal of Virology·Jonathan R GroverAkira Ono
Dec 10, 2016·RNA·Gabrielle C ToddAkira Ono

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