Sequence-specific modification of mouse genomic DNA mediated by gene targeting techniques

Cytogenetic and Genome Research
F Sangiuolo, G Novelli

Abstract

The major impact of the human genome sequence is the understanding of disease etiology with deduced therapy. The completion of this project has shifted the interest from the sequencing and identification of genes to the exploration of gene function, signalling the beginning of the post-genomic era. Contrasting with the spectacular progress in the identification of many morbid genes, today therapeutic progress is still lagging behind. The goal of all gene therapy protocols is to repair the precise genetic defect without additional modification of the genome. The main strategy has traditionally been focused on the introduction of an expression system designed to express a specific protein, defective in the transfected cell. But the numerous deficiencies associated with gene augmentation have resulted in the development of alternative approaches to treat inherited and acquired genetic disorders. Among these one is represented by gene repair based on homologous recombination (HR). Simply stated, the process involves targeting the mutation in situ for gene correction and for restoration of a normal gene function. Homologous recombination is an efficient means for genomic manipulation of prokaryotes, yeast and some lower eukaryotes. ...Continue Reading

Citations

May 22, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Peter L OliverKay E Davies
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Federica SangiuoloGiuseppe Novelli
Dec 23, 2009·International Journal of Pharmaceutics·Hiroyuki KamiyaHideyoshi Harashima
Jan 22, 2005·Trends in Biotechnology·Alexander A VolodinR Daniel Camerini-Otero
Jul 23, 2008·Biochemistry·Hiroyuki TsuchiyaHiroyuki Kamiya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Anatomical Science International
Xiao LiuChizuka Ide
Current Opinion in Plant Biology
Moez Hanin, Jerzy Paszkowski
Current Opinion in Genetics & Development
Min Gong, Yikang S Rong
© 2022 Meta ULC. All rights reserved