Sequence-specific recognition but position-dependent cleavage of two distinct telomeres by the Borrelia burgdorferi telomere resolvase, ResT

Molecular Microbiology
Yvonne TourandGeorge Chaconas

Abstract

An unusual feature of bacteria in the genus Borrelia (causative agents of Lyme disease and relapsing fever) is a segmented genome consisting of multiple linear DNA molecules with covalently closed hairpin ends, known as telomeres. The hairpin telomeres are generated by a DNA breakage and reunion process (telomere resolution) promoted by ResT, an enzyme using an active site related to that of tyrosine recombinases and type IB topoisomerases. In this study, we define the minimal sequence requirements for a functional telomere and identify specific basepairs that appear to be important for telomere resolution. In addition, we show that the two naturally occurring and distinct telomere spacings found in B. burgdorferi can both be efficiently processed by ResT. This flexibility for substrate utilization by ResT supports the argument for a single telomere resolvase in Borrelia. Furthermore, although telomere recognition requires sequence specificity in part of the substrate, DNA cleavage is instead position dependent and occurs at a fixed distance from the axis of symmetry and the conserved sequence of box 3 in the different replicated telomere substrates. This positional dependence for DNA cleavage has not been observed previously f...Continue Reading

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Citations

Jun 30, 2009·The Journal of Biological Chemistry·Tara J Moriarty, George Chaconas
Jul 15, 2009·Antimicrobial Agents and Chemotherapy·Georgia Lefas, George Chaconas
Aug 29, 2006·Journal of Bacteriology·Yvonne TourandGeorge Chaconas
May 20, 2004·Journal of Bacteriology·Rebecca ByramPatricia Rosa
Jun 19, 2004·Journal of Bacteriology·Wai Mun HuangSherwood Casjens
Apr 28, 2006·Journal of Virology·C L AfonsoD L Rock
Sep 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Troy Bankhead, George Chaconas
Jun 12, 2010·Annual Review of Microbiology·George Chaconas, Kerri Kobryn
Oct 22, 2005·Molecular Microbiology·George Chaconas
Sep 27, 2006·Molecular Microbiology·Troy BankheadGeorge Chaconas
Jan 6, 2005·Plasmid·Philip E StewartPatricia A Rosa
Mar 6, 2004·Journal of Molecular Biology·Wai Mun HuangSherwood Casjens
May 27, 2015·Nucleic Acids Research·Danica LucyshynKerri Kobryn
Dec 1, 2004·Annual Review of Genetics·Stephen D Bentley, Julian Parkhill
Mar 24, 2015·PloS One·Wolfgang SchülerUrban Lundberg
Jan 8, 2020·BMC Genomics·Konstantin V KuleshovAlexander E Platonov
Jun 25, 2015·Microbiology Spectrum·Kerri Kobryn, George Chaconas

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