Sequence-specific targeting of MSL complex regulates transcription of the roX RNA genes

The EMBO Journal
X BaiMitzi I Kuroda

Abstract

In Drosophila, dosage compensation is controlled by the male-specific lethal (MSL) complex consisting of at least five proteins and two noncoding RNAs, roX1 and roX2. The roX RNAs function in targeting MSL complex to the X chromosome, and roX transgenes can nucleate spreading of the MSL complex into flanking chromatin when inserted on an autosome. An MSL-binding site (DHS, DNaseI hypersensitive site) has been identified in each roX gene. Here, we investigate the functions of the DHS using transgenic deletion analyses and reporter assays. We find that MSL interaction with the DHS counteracts constitutive repression at roX1, resulting in male-specific expression of roX1 RNA. Surprisingly, the DHS is not required for initiation of cis spreading of MSL complex, instead local transcription of roX RNAs correlates with extensive spreading.

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Citations

Jul 6, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jan Larsson, Victoria H Meller
Oct 6, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Erinc Hallacli, Asifa Akhtar
Oct 6, 2005·Genes & Development·Fumika N HamadaMitzi I Kuroda
Jul 23, 2013·Genes & Development·Marcela M L SorucoErica Larschan
Aug 25, 2005·Cold Spring Harbor Symposia on Quantitative Biology·H OhM I Kuroda
Oct 10, 2007·Molecular and Cellular Biology·Kathleen A Worringer, Barbara Panning
Mar 22, 2008·Reproductive Biology and Endocrinology : RB&E·Roxani AngelopoulouPanagiota Manolakou
Feb 8, 2006·PLoS Genetics·Ina K DahlsveenPeter B Becker
Mar 23, 2012·PLoS Genetics·Chiat Koo Lim, Richard L Kelley
Aug 12, 2014·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Marcela M L Soruco, Erica Larschan
Aug 8, 2006·Trends in Biochemical Sciences·Xinxian Deng, Victoria H Meller
Aug 31, 2013·Transcription·Simona CugusiJohn C Lucchesi
Dec 19, 2006·Nature Reviews. Genetics·Tobias Straub, Peter B Becker
Dec 21, 2016·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jennifer A UrbanErica N Larschan
Nov 20, 2020·Nature·Claudia Isabelle Keller ValsecchiAsifa Akhtar

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