Sequence specificity and biochemical characterization of the RusA Holliday junction resolvase of Escherichia coli.

The Journal of Biological Chemistry
S N ChanR G Lloyd

Abstract

The RusA protein of Escherichia coli is an endonuclease that resolves Holliday intermediates in recombination and DNA repair. Analysis of its subunit structure revealed that the native protein is a dimer. Its resolution activity was investigated using synthetic X-junctions with homologous cores. Resolution occurs by dual strand incision predominantly 5' of CC dinucleotides located symmetrically. A junction lacking homology is not resolved. The efficiency of resolution is related inversely to the number of base pairs in the homologous core, which suggests that branch migration is rate-limiting. Inhibition of resolution at high ratios of protein to DNA suggests that binding of RusA may immobilize the junction point at non-cleavable sites. Resolution is stimulated by alkaline pH and by Mn2+. The protein is unstable in the absence of substrate DNA and loses approximately 80% of its activity within 1 min under standard reaction conditions. DNA binding stabilizes the activity. Junction resolution is inhibited in the presence of RuvA. This observation probably explains why RusA is unable to promote efficient recombination and DNA repair in ruvA+ strains unless it is expressed at a high level.

References

Oct 1, 1992·Molecular & General Genetics : MGG·I R TsanevaS C West
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B ConnollyS C West
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Oct 7, 1988·Cell·D R DuckettD M Lilley
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M E ChurchillN C Seeman
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S C West, A Körner
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L S Symington, R Kolodner
Jun 1, 1982·Cell·K MizuuchiR A Weisberg
Feb 1, 1984·Cell·D M Lilley, B Kemper
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SaitoH Shinagawa
Sep 15, 1995·Journal of Molecular Biology·R J Bennett, S C West
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J Bennett, S C West
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·N C Seeman, N R Kallenbach
Jul 11, 1994·Nucleic Acids Research·R ShahS C West
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·A StasiakE H Egelman
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·D M Lilley, R M Clegg
Jul 20, 1993·Journal of Molecular Biology·C A Parsons, S C West
Apr 5, 1993·Journal of Molecular Biology·P C SolaroB Kemper
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·R J Bennett, S C West
Feb 21, 1997·Journal of Molecular Biology·X YuE H Egelman

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Citations

May 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·David T Long, Kenneth N Kreuzer
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hocine W MankouriIan D Hickson
Feb 25, 2010·The Journal of Biological Chemistry·Ye Dee Tay, Leonard Wu
Oct 16, 1999·Nucleic Acids Research·G J SharplesP McGlynn
Oct 10, 2006·Nucleic Acids Research·Rachel MacmasterJohn B Rafferty
Jan 1, 1997·Annual Review of Genetics·S C West
Mar 29, 2008·PLoS Genetics·Zeynep BaharogluBénédicte Michel
Oct 29, 2010·Journal of Nucleic Acids·Rihito MoritaSeiki Kuramitsu
Feb 22, 2005·Molecular Microbiology·Fiona A CurtisGary J Sharples
Nov 10, 2004·DNA Repair·Qin WenRobert G Lloyd
Jun 30, 2004·Journal of Molecular Biology·Gary J SharplesEdward L Bolt
Jul 6, 2001·Journal of Molecular Biology·E L BoltG J Sharples
Feb 8, 2005·The Journal of Biological Chemistry·Geoffrey S BriggsRobert G Lloyd
Apr 5, 2008·The EMBO Journal·Laura RoseaulinBenoit Arcangioli
Jan 21, 2006·The EMBO Journal·Zeynep BaharogluBénédicte Michel
Apr 1, 2006·Journal of Molecular Recognition : JMR·Patricia A KhuuP Shing Ho
Jun 27, 2002·The Journal of Biological Chemistry·Claudette L DoeMatthew C Whitby
Nov 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Aishwarya DevarajSteven D Goodman
Dec 18, 1998·The Journal of Biological Chemistry·M C Whitby, J Dixon
May 16, 2003·The Journal of Biological Chemistry·Jong Sook Ahn, Matthew C Whitby
Oct 9, 2013·The Journal of Biological Chemistry·M Rebecca GlineburgF Brad Johnson
Oct 31, 2012·Journal of Cell Science·Amélie RodrigueJean-Yves Masson
May 26, 1998·Journal of Molecular Biology·M J Giraud-Panis, D M Lilley

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