PMID: 2499688Apr 1, 1989Paper

Sequence variability in three wheat germ agglutinin isolectins: products of multiple genes in polyploid wheat

Journal of Molecular Evolution
C S Wright, N V Raikhel

Abstract

Three highly homologous wheat germ isolectins (95-97%) are distinct gene products in hexaploid wheat. The amino acid sequences of two of these [wheat germ agglutinin 1 (WGA1) and 2 (WGA2)] are compared with sequence data derived from a complementary DNA (cDNA) clone for the third isolectin (WGA3). This comparison includes three corrections to earlier amino acid sequence data of both WGA1 and WGA2 at positions 109 (from Ser to Phe), 134 (from Gly to Lys), and 150 (from Gly to Trp). These reassignments are based on new results from crystal structure refinement and amino acid sequence data of WGA1, as well as the recently determined nucleotide sequence of WGA3. In addition, the C-terminal residue of WGA1 has been revised to Gly171 and now differs from WGA2 (Ala171). Four other positions, Asn9, Ala53, Gly119, and Ser123, at which WGA1 and WGA2 are identical but differ from the DNA sequence of WGA3, were also reinvestigated by amino acid sequencing techniques and confirmed. Variability among the three isolectins is observed at a total of 10 sequence positions: 9, 53, 56, 59, 66, 93, 109, 119, 123, and 171. Pairwise comparisons indicate that WGA3 deviates to a much larger extent from WGA1 (at eight positions) and from WGA2 (at seven ...Continue Reading

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Citations

Jan 1, 1991·Developmental Genetics·N V Raikhel, D R Lerner
Nov 1, 1989·Plant Molecular Biology·J J Smith, N V Raikhel
Jan 1, 1996·Journal of Protein Chemistry·C CarusoV Buonocore
Jan 1, 1991·The Plant Cell·M J Chrispeels, N V Raikhel
Dec 15, 1992·European Journal of Biochemistry·H NagahoraY Jigami
Jun 11, 2016·Food Chemistry·Begoña Martín-FernándezIsabel Mafra
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·H T Wright
Nov 8, 2001·DNA Sequence : the Journal of DNA Sequencing and Mapping·I J KimW I Chung
Apr 1, 1991·Cell Biophysics·D G MenterG L Nicolson
Feb 1, 1992·Biological Chemistry Hoppe-Seyler·J Kolberg
Feb 11, 2021·Foods·Luis E Rojas Tovar, Michael G Gänzle
Oct 5, 1989·Journal of Molecular Biology·C S Wright

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