PMID: 417344Apr 1, 1978

Sequence variation among heavy chains from inulin-binding myeloma proteins

Proceedings of the National Academy of Sciences of the United States of America
M VranaM Potter

Abstract

The entire sequences of the variable region of four heavy chains from BALB/c inulin-binding myeloma proteins have been determined. Among the four proteins there are six amino acid differences, all of which occur in the framework portion of the variable region. All of the six amino acid substitutions can be explained by single base mutations at the DNA level. The pattern of diversity in these proteins is compared to a previously reported group of heavy chains from phosphorylcholine-binding myeloma proteins. Unlike the phosphorylcholine-binding proteins, which (with the exception of two that are identical) have size and sequence differences in their complementarity regions, the inulin-binding heavy chains all have identical complementarity regions with H3 being extremely short. The pattern of variation observed in the anti-inulin heavy chains appears to be most easily explained by a somatic mutation mechanism. However, because none of the substitutions occur in complementarity-determining regions, they presumably would have no selective advantage and would not alter binding specificity. These proteins have further been shown to have crossreacting antigenic determinants (idiotypes). Five of the six sequence differences observed oc...Continue Reading

References

Nov 2, 1976·The Journal of Experimental Medicine·S Rudikoff, J L Claflin
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·M PotterE B Mushinski
Jun 1, 1977·Immunochemistry·T S LieuF W Tischendorf
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·M G Weigert, R Riblet
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·S TonegawaR Schuller
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Rudikoff, M Potter
Jul 11, 1975·The Journal of Experimental Medicine·R LiebermanM Vrana
Jun 22, 1971·Biochemistry·S H Bridges, J R Little
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R J PoljakF Saul
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M SegalD R Davies

Citations

Sep 1, 1990·Journal of Clinical Immunology·C BonaG Kelsoe
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D J McKeanM Potter
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N Gough, O Bernard
Jan 1, 1983·The Journal of Experimental Medicine·S H ClarkeS Rudikoff
Jan 1, 1984·The Journal of Experimental Medicine·R M PerlmutterL Hood
Nov 28, 1978·Biochemistry·D J McKean, R A Maurer
Jan 1, 1981·Immunological Reviews·J M AdamsS Cory

Related Concepts

Binding Sites, Antibody
Cistron
Framework Region, Immunoglobulin
Immunoglobulin Heavy Chain Subgroup VH-III
Inulin
Myeloma Proteins
Peptide Fragments
Phosphorylcholine

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