PMID: 2110661Apr 25, 1990Paper

Sequence variation in transcription factor IIIA

Nucleic Acids Research
C J Gaskins, J S Hanas

Abstract

Previous studies characterized macromolecular differences between Xenopus and Rana transcription factor IIIA (TFIIIA) (Gaskins et al., 1989, Nucl. Acids Res. 17, 781-794). In the present study, cDNAs for TFIIIA from Xenopus borealis and Rana catesbeiana (American bullfrog) were cloned and sequenced in order to gain molecular insight into the structure, function, and species variation of TFIIIA and the TFIIIA-type zinc finger. X. borealis and R. catesbeiana TFIIIAs have 339 and 335 amino acids respectively, 5 and 9 fewer than X. laevis TFIIIA. X. borealis TFIIIA exhibited 84% sequence homology (55 amino acid differences) with X. laevis TFIIIA and R. catesbeiana TFIIIA exhibited 63% homology (128 amino acid changes) with X. laevis TFIIIA. This sequence variation is not random; the C-terminal halves of these TFIIIAs contain substantially more non-conservative changes than the N-terminal halves. In particular, the N-terminal region of TFIIIA (that region forming strong DNA contacts) is the most conserved and the C-terminal tail (that region involved in transcription promotion) the most divergent. Hydropathy analyses of these sequences revealed zinc finger periodicity in the N-terminal halves, extreme hydrophilicity in the C-termina...Continue Reading

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Citations

Jan 1, 1991·Progress in Biophysics and Molecular Biology·B S Shastry
Jul 31, 2003·Journal of Molecular Biology·Deborah B Schulman, David R Setzer
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N A Woychik, R A Young
Apr 25, 2002·Nucleic Acids Research·Nicholas Polakowski, Marvin R Paule
Jun 28, 2002·Nucleic Acids Research·Deborah B Schulman, David R Setzer
Nov 13, 2012·Biochimica Et Biophysica Acta·Elodie LayatSylvette Tourmente
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·R T NolteR S Brown
Jul 27, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Naofumi MiwaSatoru Kawamura
Jul 10, 2003·The Plant Journal : for Cell and Molecular Biology·Catherine CloixSylvette Tourmente
Jul 10, 1998·Biochimica Et Biophysica Acta·J S HanasJ Q Wickham
Dec 1, 1993·Molecular and Cellular Biology·X Mao, M K Darby
Jun 5, 1992·Journal of Molecular Biology·C W Stephen, D P Lane

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