PMID: 9171401Jun 1, 1997Paper

Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa

Journal of Bacteriology
J L RaeI L Lamont

Abstract

A mutant of Pseudomonas aeruginosa, OT2100, which appeared to be defective in the production of the fluorescent yellow-green siderophore pyoverdine had been isolated previously following transposon mutagenesis (T. R. Merriman and I. L. Lamont, Gene 126:17-23, 1993). DNA from either side of the transposon insertion site was cloned, and the sequence was determined. The mutated gene had strong identity with the dihydrolipoamide acetyltransferase (E2) components of pyruvate dehydrogenase (PDH) from other bacterial species. Enzyme assays revealed that the mutant was defective in the E2 subunit of PDH, preventing assembly of a functional complex. PDH activity in OT2100 cell extracts was restored when extract from an E1 mutant was added. On the basis of this evidence, OT2100 was identified as an aceB or E2 mutant. A second gene, aceA, which is likely to encode the E1 component of PDH, was identified upstream from aceB. Transcriptional analysis revealed that aceA and aceB are expressed as a 5-kb polycistronic transcript from a promoter upstream of aceA. An intergenic region of 146 bp was located between aceA and aceB, and a 2-kb aceB transcript that originated from a promoter in the intergenic region was identified. DNA fragments upstr...Continue Reading

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Citations

May 5, 2000·Molecular Plant-microbe Interactions : MPMI·D CabanesJ Batut
Oct 16, 1999·European Journal of Biochemistry·A F HengeveldA de Kok
Jul 10, 1998·Biochimica Et Biophysica Acta·A de KokA H Westphal
Jul 10, 1998·Biochimica Et Biophysica Acta·U NevelingH Sahm

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