Sequences and expression patterns of alkaline phosphatase isozymes in preattachment bovine embryos and the adult bovine

Molecular Reproduction and Development
K McDougallA Hahnel

Abstract

We report the cloning and partial sequences of two novel bovine tissue-specific alkaline phosphatase (AP) isozymes (TSAP2 and TSAP3) from in vitro-produced bovine blastocysts. Using a reverse-transcribed polymerase chain reaction (RT-PCR)-based assay for mRNA expression and in vitro-produced preattachment bovine embryos, TSAP2 mRNA was detected first at the four-cell stage prior to the major burst of embryonic transcription in cattle and TSAP3 at the eight-cell stage with the major burst in transcription. Furthermore, the transcription of TSAP2 and TSAP3 displays a curious "on-off" pattern during early cleavages between 40 and 120 hr after insemination. Activity of bovine AP, measured by an azo-dye coupling technique, indicates that at least one AP isozyme is functional in oocytes and embryos throughout bovine preattachment development. However, maternal and embryonic-derived AP activity may have different cell-surface distributions. This novel expression pattern of the bovine AP isozymes could provide a useful tool for identifying and clarifying the events controlling transcription and gene expression during early embryo development.

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Citations

Mar 8, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Aus Tariq AliNigel J Crowther
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K McDougallA Hahnel
Oct 28, 2003·Molecular Reproduction and Development·Sezen AratSteven L Stice
Apr 1, 2009·Animal : an International Journal of Animal Bioscience·Z E MadejaM Switonski
Jan 28, 2003·Molecular Reproduction and Development·E M JoudreyW A King

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