PMID: 8614621Apr 1, 1996Paper

Sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in human cells: the role of transcription units

Nucleic Acids Research
D A JacksonP R Cook

Abstract

DNA sequences attaching loops of nuclear and mitochondrial DNA to underlying structures in HeLa cells have been cloned and 106 representative clones sequenced; 10 clones containing random genomic fragments served as controls. As chromatin is prone to rearrangement, care was taken to isolate sequences using 'physiological' conditions that did not create additional attachments. Comparison (by Southern blotting) of the concentration of each cloned sequence in 'total' and 'attached' fractions of DNA showed that most clones did contain attached sequences, but even highly-attached sequences were not attached in all cells in the population. Results demonstrated that 28% of clones were derived from three specific parts of the mitochondrial genome and 22% from different parts of the alu repeat. In addition, 41% of clones contained unique nuclear sequences; these contained no more of the motifs found attached to nuclear scaffolds or matrices (ie SARs or MARs) than would be expected from their base composition. No other attachment motif(s) could be identified by sequence analysis. However, Northern blotting showed that all the mitochondrial clones and 76% of clones containing unique sequences were transcribed; the degree of attachment cor...Continue Reading

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·T Igó-Kemenes, H G Zachau
Oct 15, 1992·European Journal of Biochemistry·R S Jack, H Eggert
Aug 1, 1992·Cell Biology International Reports·D A JacksonP R Cook
Jan 1, 1992·Annual Review of Biochemistry·D C Wallace
Feb 1, 1992·Molecular Microbiology·M Roberge, S M Gasser
Jan 1, 1990·Methods in Enzymology·R Staden
Dec 1, 1991·Journal of Cellular Biochemistry·R H GetzenbergD S Coffey
Jan 1, 1991·Annual Review of Cell Biology·D A Clayton
Oct 1, 1990·Molecular and Cellular Biology·A G MateraC W Schmid
Dec 11, 1990·Nucleic Acids Research·M A BatzerP L Deininger
Oct 1, 1989·Computer Applications in the Biosciences : CABIOS·R Staden
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Jurka, T Smith
Jan 1, 1984·Journal of Cell Science. Supplement·D A JacksonP R Cook
Jun 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P R Cook
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·G Vansant, W F Reynolds
Jul 1, 1993·Molecular and Cellular Biology·R J MaraiaG J Darlington
Jun 23, 1995·Journal of Molecular Biology·D S Prestridge
May 1, 1993·Journal of Cellular Biochemistry·T Boulikas

❮ Previous
Next ❯

Citations

Sep 6, 2000·Journal of Cellular Biochemistry·N N KhodarevA T Vaughan
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Anne Hansen ReeØystein Fodstad
Apr 20, 2013·Chemical Reviews·Argyris Papantonis, Peter R Cook
Jul 23, 2005·IUBMB Life·Francisco Martínez-Azorín
Apr 25, 2000·Nucleic Acids Research·M N Szentirmay, M Sawadogo
Mar 11, 2003·Nucleic Acids Research·Tanfis Istiaq AlamDongchon Kang
Dec 17, 2009·Nucleic Acids Research·Pierre BoeschRobert N Lightowlers
Mar 6, 2007·BMC Bioinformatics·Kenneth EvansLorenz Wernisch
May 26, 2004·BMC Biology·Francisco J IborraPeter R Cook
Dec 30, 1999·The Journal of Cell Biology·K WeipoltshammerP Hozák
Jan 1, 1997·Critical Reviews in Biotechnology·D B Archer, J F Peberdy
Dec 15, 2010·Mitochondrion·René PflugradtSabine Lutz-Bonengel
Sep 18, 2007·Mutation Research·Michal ZikanDavid Cibula
Jun 13, 2009·Journal of Cellular Biochemistry·Melita VidakovićGoran Poznanović
May 6, 2004·Annals of the New York Academy of Sciences·Tomotake KankiDongchon Kang
Nov 9, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Rosemary H C Wilson, Dawn Coverley
Apr 22, 2015·Nucleic Acids Research·Maïwen Caudron-HergerArgyris Papantonis
Oct 19, 2010·Biochimica Et Biophysica Acta·Pierre BoeschAndré Dietrich
Dec 7, 2011·Biochimica Et Biophysica Acta·Daniel F Bogenhagen
Nov 6, 2014·PloS One·Chrysostomos TornariSally J Dawson
Oct 21, 2016·PloS One·Mercedes Fernández-MorenoFrancisco J Blanco
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H W Chan
Dec 19, 2019·Nature Reviews. Genetics·Rieke Kempfer, Ana Pombo
Jun 11, 2017·Scientific Reports·Xiao-Yu ZhangChi Pui Pang
Mar 5, 2004·Journal of Cell Science·Henry H Q HengStephen A Krawetz
Oct 25, 2003·Journal of Cell Science·Peter R Cook

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.