PMID: 8604305Feb 15, 1996Paper

Sequences homologous to yeast mitochondrial and bacteriophage T3 and T7 RNA polymerases are widespread throughout the eukaryotic lineage

Nucleic Acids Research
N CermakianM W Gray

Abstract

Although mitochondria and chloroplasts are considered to be descendants of eubacteria-like endo- symbionts, the mitochondrial RNA polymerase of yeast is a nucleus-encoded, single-subunit enzyme homologous to bacteriophage T3 and T7 RNA polymerases, rather than a multi-component, eubacterial-type alpha 2 beta beta' enzyme, as encoded in chloroplast DNA. To broaden our knowledge of the mitochondrial transcriptional apparatus, we have used a polymerase chain reaction (PCR) approach designed to amplify an internal portion of phage T3/T7-like RNA polymerase genes. Using this strategy, we have recovered sequences homologous to yeast mitochondrial and phage T3/T7 RNA polymerases from a phylogenetically broad range of multicellular and unicellular eukaryotes. These organisms display diverse patterns of mitochondrial genome organization and expression, and include species that separated from the main eukaryotic line early in the evolution of this lineage. In certain cases, we can deduce that PCR-amplified sequences, some of which contain small introns, are localized in nuclear DNA. We infer that the T3/T7-like RNA polymerase sequences reported here are likely derived from genes encoding the mitochondrial RNA polymerase in the organisms ...Continue Reading

References

Jan 1, 1992·International Review of Cytology·M W Gray
Nov 20, 1992·Journal of Molecular Biology·L GrossW T McAllister
Dec 1, 1992·Journal of Molecular Evolution·F KempkenH D Osiewacz
May 1, 1990·Protein Engineering·M DelarueP Argos
Sep 1, 1989·Trends in Genetics : TIG·M W Gray
Jan 1, 1989·Annual Review of Cell Biology·M W Gray
Nov 25, 1988·Nucleic Acids Research·F Corpet
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A L GreenleafI R Lehman
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Aug 7, 1995·FEBS Letters·D A KostyukS N Kochetkov
Oct 20, 1995·Science·C M FraserJ C Venter
Dec 1, 1993·Current Opinion in Genetics & Development·M W Gray
Feb 5, 1993·Science·K M Lonergan, M W Gray
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Lerbs-Mache
Aug 1, 1994·PCR Methods and Applications·M A Frohman
Jan 1, 1995·Trends in Ecology & Evolution·G McFadden, P Gilson

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Citations

Mar 25, 2004·Protein Expression and Purification·Michio MatsunagaJudith A Jaehning
Feb 19, 2000·Current Opinion in Structural Biology·G M Cheetham, T A Steitz
Jun 14, 2002·The Plant Journal : for Cell and Molecular Biology·Boris HedtkeThomas Börner
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Katsuhiko S MurakamiLucia B Rothman-Denes
Dec 17, 2009·The Journal of Biological Chemistry·Swaroopa Paratkar, Smita S Patel
Nov 19, 2009·The Journal of Biological Chemistry·Maria SavkinaMichael Anikin
Apr 2, 2011·The Journal of Biological Chemistry·Swaroopa ParatkarSmita S Patel
Jan 1, 2009·The Journal of Biological Chemistry·Guo-Qing TangSmita S Patel
Dec 20, 2011·Genome Biology and Evolution·Estienne C SwartLaura F Landweber
Jan 22, 2013·Genome Biology and Evolution·Gertraud BurgerB Franz Lang
Mar 21, 1998·Nucleic Acids Research·M W GrayG Burger
Apr 25, 2003·Nucleic Acids Research·Gertraud BurgerStefanie Marx
Jan 20, 2006·Nucleic Acids Research·Jacinthe AzevedoSilva Lerbs-Mache
Sep 14, 2011·Nucleic Acids Research·Hajin KimTaekjip Ha
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Reinhold G HerrmannChristian Schmitz-Linneweber
Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Adrian C BarbrookSarah J Tarr
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·G M CheethamT A Steitz
May 20, 1999·The Plant Cell·C C ChangD B Stern
Aug 26, 1999·The Journal of Eukaryotic Microbiology·B F LangG Burger
Mar 31, 2010·Annual Review of Biochemistry·Nils-Göran Larsson
Jan 1, 1997·Annual Review of Biochemistry·G S Shadel, D A Clayton
May 26, 1999·Critical Reviews in Microbiology·D CorsaroM Valassina
Nov 12, 2013·FEBS Letters·Tingjian Chen, Floyd E Romesberg
Nov 26, 2014·BMC Evolutionary Biology·Sivakumar KannanEugene V Koonin
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Kyoko BabaRishikesh P Bhalerao
Oct 28, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Alexander A Tokmakov, Yasuo Fukami
Nov 26, 2009·The Plant Journal : for Cell and Molecular Biology·Uwe RichterThomas Börner
Nov 9, 2004·Current Opinion in Genetics & Development·Vincent Daubin, Howard Ochman

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