PMID: 9422345Jan 9, 1998Paper

Sequences in the amino termini of GABA rho and GABA(A) subunits specify their selective interaction in vitro

Journal of Neurochemistry
A S HackamG R Cutting

Abstract

Molecular cloning has revealed that there are six classes of subunits capable of forming GABA-gated chloride channel receptors. GABA(A) receptors are composed of alpha, beta, gamma, delta, and epsilon/chi subunits, whereas GABA(C) receptors appear to contain rho subunits. However, retinal cells exhibiting GABA(C) responses express alpha, beta, and rho subunits, raising the possibility that GABA(C) receptors may be a mixture of subunit classes. Using in vitro translated protein, we determined that human GABA(A) receptor subunits alpha1, alpha5, and beta1 did not coimmunoprecipitate with full-length rho1, rho2, or the N-terminal domain of rho1 that contains signals for rho-subunit interaction. To explore the molecular mechanism underlying these apparently exclusive combinations, chimeric subunits were created and tested for interaction with the wild-type subunits. Transfer of the N terminus of beta1 to rho1 created a beta1rho1 chimera that coimmunoprecipitated with the alpha1 subunit but not with the rho2 subunit. Furthermore, exchanging the N terminus of the rho1 subunit with the corresponding region of beta1 produced a rho1beta1 chimera that interfered with rho1 receptor expression in Xenopus oocytes, whereas the full-length be...Continue Reading

Citations

Oct 11, 2001·Biological Chemistry·R Enz
Nov 20, 2002·Journal of Neurochemistry·Jesper Nymann-AndersenRichard W Olsen
Mar 25, 2011·Future Medicinal Chemistry·Clarissa K L NgMary Chebib
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Dec 10, 2002·The Journal of Biological Chemistry·Karen BollanChristopher N Connolly
Sep 10, 1999·The Journal of Biological Chemistry·S SrinivasanA J Tobin
Apr 3, 2001·Nature Reviews. Neuroscience·S J Moss, T G Smart
Oct 9, 1999·Journal of Neurophysiology·M PasternackM Schmidt
Mar 28, 2003·The Journal of Biological Chemistry·Katia Gamel-DidelonArtur Mayerhofer
Oct 28, 2014·ACS Chemical Neuroscience·Lik-Wei WongBrett A Cromer

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