Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes

The Journal of General Virology
Sandrine CrochuXavier de Lamballerie

Abstract

Flavivirus-related sequences have been discovered in the dsDNA genome of Aedes albopictus and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the Aedes albopictus C6/36 cell line, an open reading frame (ORF) of 1557 aa with protease/helicase and polyprotein processing domains characteristic of flaviviruses was identified. It is closely related to NS1-NS4A genes of the Cell Fusing Agent and Kamiti River virus and the corresponding mRNAs were detected. Integrated sequences homologous to the envelope, NS4B and polymerase genes of flaviviruses were identified. Overall, approximately two-thirds of a flavivirus-like genome were characterized. In the Aedes aegypti A20 cell line, a 492 aa ORF related to the polymerase of the Cell Fusing Agent and Kamiti River virus was identified. These flavivirus-related integrated DNA sequences were detected in laboratory-bred and wild Aedes albopictus and Aedes aegypti mosquitoes, demonstrating that their discovery is not an artefact resulting from the manipulation of mosquito cell lines, since they exist under natural conditions. This finding has ma...Continue Reading

References

Aug 7, 1975·Nature·V M Zhdanov
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1974·Archiv für die gesamte Virusforschung·V M Zhdanov, M I Parfanovich
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Dec 31, 1997·Nature·P KlenermanR M Zinkernagel
Jul 31, 2001·Molecular Phylogenetics and Evolution·R B Simmons, S J Weller
Dec 26, 2001·Bioinformatics·S KumarM Nei
Sep 19, 2002·The Journal of General Virology·X de LamballerieE A Gould
Jan 10, 2003·Molecular Biology and Evolution·S Susanna TwiddyAndrew Rambaut

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Citations

Nov 18, 2005·Archives of Virology·E R Chare, E C Holmes
Apr 16, 2010·Marine Biotechnology·T W Flegel, Kallaya Sritunyalucksana
Mar 8, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Yi-Ying ChenShyan-Song Chiou
Nov 21, 2007·Vector Borne and Zoonotic Diseases·G MoureauX de Lamballerie
Oct 31, 2008·Vector Borne and Zoonotic Diseases·C ArandaA Tenorio
Jun 3, 2009·Vector Borne and Zoonotic Diseases·María-Paz Sánchez-SecoAntonio Tenorio
Apr 8, 2010·Vector Borne and Zoonotic Diseases·Mattia CalzolariMichele Dottori
Oct 26, 2011·Vector Borne and Zoonotic Diseases·Ana VázquezAntonio Tenorio
Aug 14, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Masayuki HorieKeizo Tomonaga
Jun 14, 2013·MBio·Anne KoppSandra Junglen
Nov 19, 2011·BMC Evolutionary Biology·Derek J TaylorJeremy A Bruenn
Dec 22, 2009·BMC Biology·Derek J Taylor, Jeremy Bruenn
Jan 14, 2010·BMC Biology·Eugene V Koonin
Jul 3, 2013·Virology Journal·Birgit NikolayAmadou A Sall
Feb 16, 2008·Virology Journal·Stephen J Seligman
Oct 12, 2010·PLoS Biology·Clément Gilbert, Cédric Feschotte
Dec 3, 2010·PLoS Genetics·Aris Katzourakis, Robert J Gifford
Aug 14, 2008·PLoS Neglected Tropical Diseases·Asif M KhanJ Thomas August
Apr 30, 2009·PloS One·Qi Ying KooJ Thomas August
Jul 22, 2005·Emerging Infectious Diseases·Remi N CharrelXavier de Lamballerie
Jul 8, 2011·The American Journal of Tropical Medicine and Hygiene·Bethany G BollingCarol D Blair
Nov 12, 2013·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Lars HarmsMagnus Lucassen
Nov 3, 2014·Annual Review of Virology·Nikolaus OsterriederBenedikt B Kaufer
Mar 15, 2014·Memórias do Instituto Oswaldo Cruz·Sandra Regina MaruyamaIsabel Kinney Ferreira de Miranda-Santos
Nov 22, 2014·Viruses·Shuzhen SimGeorge Dimopoulos
May 7, 2011·Expert Review of Molecular Diagnostics·Ross Thomas BarnardErnest A Gould
Nov 15, 2014·International Journal of Environmental Research and Public Health·Hugo C OsórioMaria J Alves
Oct 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Guang ChenAnthony A James
Sep 18, 2015·Viruses·Bethany G BollingNikos Vasilakis
Oct 15, 2013·Journal of Molecular Biology·Megan B KingsolverRichard W Hardy
May 4, 2011·Journal of Invertebrate Pathology·Steven M Valles, Blake Bextine

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