PMID: 9449282Feb 4, 1998Paper

Sequences of homologous beta-lactamases from clinical isolates of Serratia marcescens with different substrate specificities

Antimicrobial Agents and Chemotherapy
N MatsumuraS Mitsuhashi

Abstract

Genes for two group 1 beta-lactamases, SRT-1 and SST-1, were sequenced. These beta-lactamases were produced by clinical isolates of Serratia marcescens, isolates GN16694 and GN19450, respectively. The resulting enzymes were 96% identical. SRT-1 hydrolyzed oxyimino cephalosporins, but SST-1 hardly hydrolyzed them. At residue 213 in the third motif, which is conserved among group 1 beta-lactamases, SRT-1 and SST-1 had Lys and Glu, respectively. By site-directed mutagenesis, the substitution of Glu by Lys at residue 213 in SST-1 resulted in an enzyme that hydrolyzed oxyimino cephalosporins.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C C Sanders
Jan 1, 1991·Annual Review of Microbiology·J M Ghuysen
Jan 1, 1991·Antimicrobial Agents and Chemotherapy·M WatanabeS Mitsuhashi
Feb 22, 1990·European Journal of Biochemistry·K TsukamotoT Sawai
May 2, 1986·European Journal of Biochemistry·F Lindberg, S Normark
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B Jaurin, T Grundström
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·K BushA A Medeiros
Mar 17, 1995·The Journal of Biological Chemistry·M NukagaT Sawai
Sep 1, 1995·Antimicrobial Agents and Chemotherapy·N Matsumura, S Mitsuhashi

❮ Previous
Next ❯

Citations

Apr 28, 2005·Antimicrobial Agents and Chemotherapy·Susanna K P LauKwok-yung Yuen
Jun 26, 2007·The Journal of Antimicrobial Chemotherapy·Hedi MammeriPatrice Nordmann
Mar 4, 2008·The FEBS Journal·Catherine MichauxJohan Wouters
Mar 5, 2009·Antimicrobial Agents and Chemotherapy·José-Manuel Rodríguez-MartínezPatrice Nordmann
Mar 31, 2017·Annals of Clinical Microbiology and Antimicrobials·Christopher RayNorberto González-Juarbe
Mar 23, 2018·The Journal of Antimicrobial Chemotherapy·Rebekka KohlmannSören G Gatermann
Sep 18, 2002·Antimicrobial Agents and Chemotherapy·Richard BonnetJacques Sirot
Jan 13, 2009·Clinical Microbiology Reviews·George A Jacoby
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Hedi MammeriPatrice Nordmann
Mar 28, 2009·PLoS Pathogens·Jung Hun LeeSang Hee Lee
Aug 1, 2007·Antimicrobial Agents and Chemotherapy·Costas C PapagiannitsisVivi Miriagou
Jul 31, 2007·Future Microbiology·Patrice Nordmann, Hedi Mammeri
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·George A Jacoby
May 28, 2009·Antimicrobial Agents and Chemotherapy·Stathis D KotsakisVivi Miriagou
Jan 11, 2012·Antimicrobial Agents and Chemotherapy·Sandrine Dahyot, Hedi Mammeri

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.