Sequences within the 5' untranslated region regulate the levels of a kinetoplast DNA topoisomerase mRNA during the cell cycle.

Molecular and Cellular Biology
S G PasionD S Ray

Abstract

Gene expression in trypanosomatids appears to be regulated largely at the posttranscriptional level and involves maturation of mRNA precursors by trans splicing of a 39-nucleotide miniexon sequence to the 5' end of the mRNA and cleavage and polyadenylation at the 3' end of the mRNA. To initiate the identification of sequences involved in the periodic expression of DNA replication genes in trypanosomatids, we have mapped splice acceptor sites in the 5' flanking region of the TOP2 gene, which encodes the kinetoplast DNA topoisomerase, and have carried out deletion analysis of this region on a plasmid-encoded TOP2 gene. Block deletions within the 5' untranslated region (UTR) identified two regions (-608 to -388 and -387 to -186) responsible for periodic accumulation of the mRNA. Deletion of one or the other of these sequences had no effect on periodic expression of the mRNA, while deletion of both regions resulted in constitutive expression of the mRNA throughout the cell cycle. Subcloning of these sequences into the 5' UTR of a construct lacking both regions of the TOP2 5' UTR has shown that an octamer consensus sequence present in the 5' UTR of the TOP2, RPA1, and DHFR-TS mRNAs is required for normal cycling of the TOP2 mRNA. Mu...Continue Reading

References

Jan 1, 1992·International Review of Cytology·K Stuart, J E Feagin
Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·L H JohnstonA Sugino
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J H LeBowitzS M Beverley
Jun 29, 1990·Cell·N Agabian
Jan 1, 1988·Annual Review of Microbiology·K A RyanP T Englund
Jan 1, 1986·International Review of Cytology·L Simpson
Jun 20, 1967·Biochimica Et Biophysica Acta·E W GuttesS Guttes
Jun 1, 1993·Genes & Development·J H LeBowitzS M Beverley
Jun 1, 1995·Parasitology Today·S V Graham

❮ Previous
Next ❯

Citations

Jul 9, 2010·Trends in Parasitology·Susanne Kramer, Mark Carrington
Apr 13, 2011·PloS One·Stuart K ArcherChristine Clayton
Sep 22, 2012·Annual Review of Microbiology·Robert E Jensen, Paul T Englund
May 6, 2003·The Journal of Biological Chemistry·Bidyottam MittraDan S Ray
Jun 21, 2001·Molecular and Cellular Biology·R MahmoodD S Ray
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Jan-Peter DanielsBill Wickstead
Feb 24, 2017·Journal of Biosciences·Shilpa J RaoJayantapal K Pal
Jul 25, 2020·Trends in Parasitology·Akila YagoubatYvon Sterkers
Mar 19, 2002·Molecular and Biochemical Parasitology·Emmanuel TetaudAlan H Fairlamb
Sep 1, 1997·Molecular and Biochemical Parasitology·J C Hines, D S Ray
Mar 20, 2001·Molecular and Biochemical Parasitology·R CarrilloL Simpson
Sep 23, 1997·Molecular and Biochemical Parasitology·J C Hines, D S Ray
Aug 27, 1998·Molecular and Biochemical Parasitology·J C Hines, D S Ray

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.