PMID: 9420199Jan 7, 1998Paper

Sequences within the herpesvirus-conserved pac1 and pac2 motifs are required for cleavage and packaging of the murine cytomegalovirus genome

Journal of Virology
Michael A McVoyE S Mocarski

Abstract

The DNA sequence motifs pac1 [an A-rich region flanked by poly(C) runs] and pac2 (CGCGGCG near an A-rich region) are conserved near herpesvirus genomic termini and are believed to mediate cleavage of genomes from replicative concatemers. To determine their importance in the cleavage process, we constructed a number of recombinant murine cytomegaloviruses with a second cleavage site inserted at an ectopic location within the viral genome. Cleavage at a wild-type ectopic site occurred as frequently as at the natural cleavage site, whereas mutation of this ectopic site revealed that some of the conserved motifs of pac1 and pac2 were essential for cleavage whereas others were not. Within pac1, the left poly(C) region was very important for cleavage and packaging but the A-rich region was not. Within pac2, the A-rich region and adjacent sequences were essential for cleavage and packaging and the CGCGGCG region contributed to, but was not strictly essential for, efficient cleavage and packaging. A second A-rich region was not important at all. Furthermore, mutations that prevented cleavage also blocked duplication and deletion of the murine cytomegalovirus 30-bp terminal repeat at the ectopic site, suggesting that repeat duplication ...Continue Reading

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Citations

Nov 23, 2016·BMC Genomics·Banhi BiswasPerumal Vivekanandan
Oct 22, 2008·The Journal of General Virology·Jian Ben Wang, Michael A McVoy
Jun 8, 2012·Journal of Virology·Charlotte MahietSébastien Barradeau
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·K AdelmanJ D Baines
Mar 2, 2018·ACS Infectious Diseases·Banhi BiswasPerumal Vivekanandan

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