PMID: 8955384Dec 1, 1996Paper

Sequencing and expression of a gene encoding a bile acid transporter from Eubacterium sp. strain VPI 12708

Journal of Bacteriology
D H Mallonee, P B Hylemon

Abstract

Eubacterium sp. strain VPI 12708 expresses inducible bile acid 7alpha-dehydroxylation activity via a multistep pathway. The genes encoding several of the inducible proteins involved in the pathway have been previously mapped to a bile acid-inducible (bai) operon in Eubacterium sp. strain VPI 12708. We now report the cloning, sequencing, and characterization of the baiG gene, which is part of the bai operon. The predicted amino acid sequence of the BaiG polypeptide shows significant homology to several membrane transport proteins, including sugar and antibiotic resistance transporters, which are members of the major facilitator superfamily. Hydrophilicity plots of BaiG show a high degree of similarity to class K and L TetA proteins from gram-positive bacteria, and, like these classes of TetA proteins, BaiG has 14 proposed transmembrane domains. The baiG gene was cloned into Escherichia coli and shown to confer an energy-dependent bile acid uptake activity. Primary bile acids were preferentially transported into E. coli cells expressing this gene, with at least sevenfold and fourfold increases in the uptake of cholic acid and chenodeoxycholic acid, respectively, over control reactions. Less transport activity was observed with ch...Continue Reading

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Citations

Feb 17, 1999·FEMS Microbiology Reviews·P B Hylemon, J Harder
Nov 22, 2005·Journal of Lipid Research·Jason M RidlonPhillip B Hylemon
Aug 17, 2005·FEMS Microbiology Reviews·Máire BegleyColin Hill
Apr 11, 2017·European Journal of Nutrition·Ian RowlandKieran Tuohy
Oct 19, 2017·Frontiers in Medicine·Verónica Urdaneta, Josep Casadesús
Nov 23, 2010·Applied Microbiology and Biotechnology·Bodo Philipp
Jul 31, 2013·Applied and Environmental Microbiology·Vijayakumar Somalinga, William W Mohn
Aug 12, 2014·The Journal of Membrane Biology·Jian-Shan Cai, Jin-Hong Chen
Mar 5, 2016·Gut Microbes·Jason M RidlonPhillip B Hylemon
Sep 12, 2014·Journal of Lipid Research·Paul A Dawson, Saul J Karpen
Oct 10, 2019·Gut Microbes·Jenessa A Winston, Casey M Theriot
Jun 15, 1999·Molecular Biotechnology·D H Mallonee, P B Hylemon
Aug 24, 2012·Future Microbiology·Mahfuzur R Sarker, Daniel Paredes-Sabja
Feb 5, 2008·Journal of Bacteriology·Joseph A Sorg, Abraham L Sonenshein
Dec 9, 2017·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Spencer C HarrisJason M Ridlon
Aug 30, 2000·Microbes and Infection·J S Gunn
Jun 13, 2017·Molecular Aspects of Medicine·Sarah L LongSusan A Joyce
Mar 12, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·L A ThomasR H Dowling
Feb 6, 2003·International Journal of Food Microbiology·Abelardo MargollesClara G de los Reyes-Gavilán
Apr 1, 1997·Journal of Bacteriology·D G ThanassiH Nikaido
Jul 28, 2009·Applied and Environmental Microbiology·Erika A Pfeiler, Todd R Klaenhammer
Mar 1, 1997·Applied and Environmental Microbiology·K C DoernerP B Hylemon
Jun 16, 2010·Antimicrobial Agents and Chemotherapy·Charles DarkohHerbert L DuPont
Jun 16, 2021·Microbiome·Douglas V Guzior, Robert A Quinn
Dec 17, 2020·The Journal of Infectious Diseases·Colleen M Pike, Casey M Theriot
Aug 28, 2021·Microorganisms·Franziska Maria FellerBodo Philipp
Oct 20, 2021·PLoS Pathogens·Andrea Martinez AguirreJoseph A Sorg

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