Sequencing-based counting and size profiling of plasma Epstein-Barr virus DNA enhance population screening of nasopharyngeal carcinoma

Proceedings of the National Academy of Sciences of the United States of America
W K Jacky LamY M Lo

Abstract

Circulating tumor-derived DNA testing for cancer screening has recently been demonstrated in a prospective study on identification of nasopharyngeal carcinoma (NPC) among 20,174 asymptomatic individuals. Plasma EBV DNA, a marker for NPC, was detected using real-time PCR. While plasma EBV DNA was persistently detectable in 97.1% of the NPCs identified, ∼5% of the general population had transiently detectable plasma EBV DNA. We hypothesized that EBV DNA in plasma of subjects with or without NPC may have different molecular characteristics. We performed target-capture sequencing of plasma EBV DNA and identified differences in the abundance and size profiles of EBV DNA molecules within plasma of NPC and non-NPC subjects. NPC patients had significantly higher amounts of plasma EBV DNA, which showed longer fragment lengths. Cutoff values were established from an exploratory dataset and tested in a validation sample set. Adopting an algorithm that required a sample to concurrently pass cutoffs for EBV DNA counting and size measurements, NPCs were detected at a positive predictive value (PPV) of 19.6%. This represented superior performance compared with the PPV of 11.0% in the prospective screening study, which required participants wi...Continue Reading

References

Feb 1, 1979·Journal of Virology·J E ShawC W Carter
Jan 8, 2004·Clinical Chemistry·K C Allen ChanY M Dennis Lo
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
Dec 15, 2010·Science Translational Medicine·Y M Dennis LoRossa W K Chiu
Jan 29, 2011·Chinese Journal of Cancer·Su-Mei CaoChao-Nan Qian
Sep 13, 2011·PloS One·Florent MouliereAlain R Thierry
Jun 1, 2012·Science Translational Medicine·Tim ForshewNitzan Rosenfeld
Mar 15, 2013·The New England Journal of Medicine·Sarah-Jane DawsonNitzan Rosenfeld
Mar 19, 2014·Cell Host & Microbe·Enrique A MesriKarl Munger
Mar 19, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S F LeungA T C Chan
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Stephanie C Y YuY M Dennis Lo
Nov 15, 2014·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Sarah N SmithAnita Agrawal
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Florent Mouliere, Nitzan Rosenfeld
Mar 20, 2015·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yoon-Nyun KimChang Hee Lee
Jun 18, 2015·BMC Medical Genomics·Dineika ChandranandaMelanie Bahlo
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kun SunYuk Ming Dennis Lo
Jul 20, 2016·PLoS Genetics·Hunter R UnderhillJay Shendure
Feb 16, 2017·Cancer Cell·Alberto Bardelli, Klaus Pantel
Aug 10, 2017·The New England Journal of Medicine·K C Allen ChanY M Dennis Lo
Aug 10, 2017·The New England Journal of Medicine·Richard F Ambinder

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Citations

Oct 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Peiyong JiangRossa W K Chiu
Nov 10, 2018·International Journal of Cancer. Journal International Du Cancer·Octavia RamayantiDirk Michiel Pegtel
Dec 7, 2018·F1000Research·W K Jacky Lam, Jason Y K Chan
Dec 5, 2018·Expert Review of Molecular Diagnostics·Wai Kei Jacky Lam, Kwan Chee Allen Chan
Sep 8, 2018·International Journal of Cancer. Journal International Du Cancer·Victor Ho-Fun LeeAnne Wing-Mui Lee
Feb 5, 2019·The Journal of Pathology·Wai Kei Jacky LamYuk Ming Dennis Lo
Aug 31, 2019·International Journal of Cancer. Journal International Du Cancer·Lu Ping TanUNKNOWN Malaysian Nasopharyngeal Carcinoma Study Group
Mar 20, 2020·American Society of Clinical Oncology Educational Book·Brigette B Y MaJun Ma
Apr 7, 2020·Expert Opinion on Therapeutic Targets·Valentin BalochePierre Busson
Aug 25, 2020·The Journal of Applied Laboratory Medicine·Wai Kei Jacky Lam
Feb 25, 2020·BioMed Research International·Xiaofang CuiWeiyang Li
Jun 20, 2020·Nature Reviews. Clinical Oncology·Y M Dennis Lo, W K Jacky Lam
Sep 25, 2020·British Journal of Cancer·Laura KellerKlaus Pantel
Apr 18, 2019·Annual Review of Genomics and Human Genetics·Kenna R Mills Shaw, Anirban Maitra
Nov 2, 2019·Critical Reviews in Clinical Laboratory Sciences·Eleni Tzanikou, Evi Lianidou
Jul 12, 2020·BMC Genomics·Juntang GuoJin Huang
Dec 30, 2018·Proceedings of the National Academy of Sciences of the United States of America·Lee SerpasY M Dennis Lo
Dec 8, 2020·International Journal of Cancer. Journal International Du Cancer·Geng-Hang ChenSu-Mei Cao
Mar 29, 2020·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E P HuiA T C Chan
Feb 13, 2019·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A OtandaultA R Thierry
Nov 25, 2020·Annual Review of Pathology·Jonathan C Dudley, Maximilian Diehn
Jan 26, 2021·Frontiers in Immunology·Gunnar Houen, Nicole Hartwig Trier
Jan 25, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Iuliia A PolinaKristine Y DeLeon-Pennell
May 18, 2020·International Journal of Radiation Oncology, Biology, Physics·Steven De MichinoScott V Bratman
Jun 22, 2021·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Anne W M LeeAlfio Ferlito
Mar 27, 2019·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A D KingK C A Chan
Jul 1, 2021·Hematology/oncology Clinics of North America·Harish N Vasudevan, Sue S Yom
Jul 2, 2021·Nature Reviews. Clinical Oncology·Kenneth C W WongAnthony T C Chan
Jun 24, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Justin M BurgenerScott V Bratman
Aug 11, 2021·Cancer Cytopathology·Daniel L Faden

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Methods Mentioned

BETA
biopsies
PCR
the

Software Mentioned

Perl
SOAP2
R

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