Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry

Nature Biotechnology
D J FuH Köster

Abstract

Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass spectrometric (MS) sequencing. The MS sequencing scheme is designed for high throughput and parallel sample processing, and is amenable to full automation. Reliable sequencing data were obtained using fmol sample amounts. The high resolution and accuracy of MS sequencing was demonstrated by direct sequencing of a heterozygous template.

References

Sep 1, 1994·Rapid Communications in Mass Spectrometry : RCM·K TangC H Chen
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·M T RoskeyL A Haff
Jan 1, 1996·Annual Review of Medicine·D Sidransky, M Hollstein
Oct 1, 1996·Nature Genetics·D A Clayton
Jan 1, 1996·Rapid Communications in Mass Spectrometry : RCM·S MouradianL M Smith
Sep 1, 1996·Genetic Analysis : Biomolecular Engineering·C JurinkeH Köster
Oct 1, 1996·Genetic Analysis : Biomolecular Engineering·N I TaranenkoC H Chen
Mar 1, 1997·Nature Medicine·J A Monforte, C H Becker
Jun 1, 1997·Genome Génome / Conseil National De Recherches Canada·J O OlufowoteS R McCouch
Dec 31, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·D P LittleH Köster
Sep 1, 1996·Nature Biotechnology·H KösterC R Cantor

❮ Previous
Next ❯

Citations

Nov 1, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·UNKNOWN CODATA Task Group on biological macromolecules and colleagues. Committee on Data for Science and Technology of the Internati
Apr 1, 2003·Mass Spectrometry Reviews·Jörg Tost, Ivo G Gut
Apr 15, 2005·Mutation Research·John R EdwardsJingyue Ju
Feb 1, 2000·Trends in Biotechnology·T J Griffin, L M Smith
Mar 11, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J J Going, B A Gusterson
Mar 20, 1999·Genetic Analysis : Biomolecular Engineering·J H GraberC R Cantor
Jun 22, 2000·Molecular Medicine Today·P E JacksonJ D Groopman
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·James J WaltersA Fox
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sascha Sauer, Ivo G Gut
Feb 4, 2010·Accounts of Chemical Research·Jia GuoJingyue Ju
Jul 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jia GuoJingyue Ju
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·K TangH Köster
Oct 12, 2000·Nucleic Acids Research·E NordhoffH Lehrach
Nov 3, 2001·Nucleic Acids Research·J R EdwardsJ Ju
May 25, 2004·Nucleic Acids Research·Emil P Kartalov, Stephen R Quake
Feb 6, 2004·Genome Research·Hameer RuparelJingyue Ju
Nov 10, 2001·Annual Review of Genomics and Human Genetics·K U Mir, E M Southern
Nov 28, 2001·Pharmacogenomics·L A LarsenP S Andersen
Jan 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zengmin LiJingyue Ju
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jingyue JuNicholas J Turro
Oct 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jian WuJingyue Ju
Apr 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tae Seok SeoJingyue Ju
Apr 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tae Seok SeoJingyue Ju
Dec 2, 2006·Journal of Biochemical and Biophysical Methods·Sascha Sauer
Mar 16, 2005·Clinical Biochemistry·Jörg Tost, Ivo Glynne Gut
Sep 29, 2004·Mutation Research·Max KällerJoakim Lundeberg
Sep 6, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sascha Sauer
Jul 12, 2001·Annual Review of Biomedical Engineering·A Marziali, M Akeson
Aug 7, 2001·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·T Bonk, A Humeny
Nov 25, 2003·Nature Reviews. Genetics·Sobin KimJingyue Ju
Dec 16, 1998·Nature Biotechnology·P RossL Haff
Aug 19, 2005·European Journal of Mass Spectrometry·Julia GrabitzkiGünter Lochnit
Dec 31, 2005·BioTechniques·Jun WangSoren Germer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved