PMID: 11342216May 9, 2001Paper

Sequencing, expression, characterisation and phylogeny of the ADP-dependent phosphofructokinase from the hyperthermophilic, euryarchaeal Thermococcus zilligii

Biochimica Et Biophysica Acta
Ron S RonimusHugh W Morgan

Abstract

The full-length gene encoding the ADP-dependent phosphofructokinase (PFK) from the euryarchaeal Thermococcus zilligii was cloned, using degenerate primer polymerase chain reaction (PCR) combined with inverse-PCR techniques, and ultimately expressed in Escherichia coli. The expressed enzyme was biochemically characterised and found to be similar to the native enzyme for most properties examined. Sequence database searches suggest that this unique ADP-PFK possesses a limited phylogenetic distribution with homologues being found only in the other euryarchaeta Methanococcus jannaschii, Methanosarcina mazei and closely related members of the order Thermococcales. A phylogenetic analysis suggests that a single ancestral gene diverged to form the glucokinase and PFK lineages of this unique sequence family. Thus, the PFK reaction, one of the defining enzymatic activities of the Embden-Meyerhof pathway, can now be represented by three separate sequence families, the well-known PFKA family exemplified by the primary E. coli ATP-PFK (E.C. 2.7.1.11) and its associated ATP- and pyrophosphate-dependent PFKs (EC.2.7.1.90), the PFKB family (E. coli PFK 2 encoded by the pfkB gene and its homologues) and the ADP-PFKs of the Euryarchaeota reporte...Continue Reading

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Citations

Apr 5, 2001·FEMS Microbiology Letters·S GebhardH W Morgan
Nov 22, 2001·Journal of Bacteriology·C H VerheesW M de Vos
Feb 6, 2004·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Haruhiko SakurabaToshihisa Ohshima
Jun 24, 2009·IUBMB Life·Victoria Guixé, Felipe Merino
May 27, 2011·Current Opinion in Microbiology·Takaaki Sato, Haruyuki Atomi
Jun 28, 2011·Acta Biochimica Et Biophysica Sinica·Qing ZhangGuoping Zhao
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Christopher BräsenBettina Siebers

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