PMID: 2106685Mar 1, 1990Paper

Sequencing of peptides and proteins with blocked N-terminal amino acids: N-acetylserine or N-acetylthreonine

Proceedings of the National Academy of Sciences of the United States of America
D WellnerB L Horecker

Abstract

Many proteins cannot be directly sequenced by Edman degradation because they have a blocked N-terminal residue. A method is presented for deblocking such proteins when the N-terminal residue is N-acetylserine (which occurs frequently in eukaryotic proteins) or N-acetylthreonine. The method has been applied successfully to the determination of the N-terminal amino acid sequence of human, bovine, and rat parathymosins. Prothymosin alpha and other blocked proteins and peptides were also readily deblocked and sequenced by this procedure. It is proposed that the mechanism of the deblocking reaction involves an acid-catalyzed N----O shift of the acetyl group followed by a beta-elimination.

References

Feb 15, 1989·Biochemical and Biophysical Research Communications·M ClintonB L Horecker
Sep 15, 1988·Biochemical and Biophysical Research Communications·C PanneerselvamB L Horecker
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T KomiyamaB L Horecker
Aug 29, 1986·Biochemical and Biophysical Research Communications·W M JonesJ M Manning
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N TakahashiF W Putnam
Nov 4, 1985·European Journal of Biochemistry·B PerssonH Jörnvall
May 7, 1974·Biochemical and Biophysical Research Communications·S NakamuraK Konishi
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T L LowA L Goldstein
Apr 1, 1958·Biochimica Et Biophysica Acta·K NARITA
Dec 23, 1961·Nature·E MARGOLIASHH TUPPY

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Citations

Jun 1, 1993·Journal of Protein Chemistry·J D Hockenhull-JohnsonD A Walz
Jan 1, 1992·The International Journal of Biochemistry·K K Han, A Martinage
Apr 22, 1992·Biochimica Et Biophysica Acta·K Muramoto, H Kamiya
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C H PohP Gopalakrishnakone
May 26, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Nobuhiro Kan-NoMinoru Sato
Apr 28, 2004·Insect Biochemistry and Molecular Biology·Rashmi SharmaHiroaki Noda
Mar 18, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ren-Huai Huang, Da-Cheng Wang
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·L A FerreiraR B Zingali
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P KwiterovichM Miller
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S M HanashJ T Watson
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·R E SobelC D Allis
Dec 1, 1991·Journal of Protein Chemistry·J D Hockenhull-JohnsonD A Walz
Nov 1, 1994·Journal of Protein Chemistry·M V SousaL Morhy
Jul 1, 2009·Fish & Shellfish Immunology·Anacleto M Argayosa, Yuan C Lee
Sep 1, 2015·Allergologia et immunopathologia·C M Landa-PinedaC A Reyes-López
Apr 11, 2000·The Journal of Allergy and Clinical Immunology·H D ShenS H Han
Jun 11, 2005·Biotechnology and Bioengineering·Anne IncampsEric Quéméneur
Nov 23, 2005·The Journal of Biological Chemistry·Séverine ZirahSylvie Rebuffat
Dec 14, 2004·American Journal of Physiology. Cell Physiology·Nicole D SmallwoodCarole M Liedtke
May 15, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·L BetancourtY Shimonishi
Jun 6, 2020·Pharmacology Research & Perspectives·Stefan SchreiberTakafumi Iwura
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hun Sik KimSun Chang Kim
Apr 23, 2008·Current Protocols in Protein Science·E FowlerF Wold

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