Sequencing of the putative DNA helicase-encoding gene of the Bombyx mori nuclear polyhedrosis virus and fine-mapping of a region involved in host range expansion

Gene
S G Kamita, S Maeda

Abstract

The complete sequence of a 3666-nucleotide (nt) open reading frame (ORF) and its flanking regions (58.1-62.1 map units (m.u.) from Bombyx mori nuclear polyhedrosis virus (BmNPV)) was determined. This ORF, BmNPV dnahel, encoded a predicted protein of 143623 Da which possessed seven consensus motifs found in proteins which unwind duplex DNAs, indicating that it is a DNA helicase. A 572-bp SacI-HindIII fragment, BmScH, that was previously shown to expand the host range of Autographa californica NPV (AcNPV) following homologous recombination [Maeda et al. (1993) J. Virol. 67, 6234-6238], was localized within BmNPV dnahel. By cotransfection experiments, two adjacent nt (A and T) that appeared to be the minimal essential sequence necessary to expand the host range of AcNPV, were mapped within BmScH. These adjacent nt encoded a single amino acid difference between BmNPV (Asp) and AcNPV (Ser).

Citations

Oct 16, 2009·Applied Microbiology and Biotechnology·Tatsuya KatoEnoch Y Park
Feb 25, 2014·Insect Biochemistry and Molecular Biology·Liang Jiang, Qingyou Xia
Mar 20, 2013·Virus Research·Genki IshiharaSusumu Katsuma
Mar 21, 2006·Biochemical and Biophysical Research Communications·Masayuki KitajimaHiroshi Takaku
Feb 16, 2006·Journal of Virological Methods·Vipin Kumar DeoEnoch Y Park
Mar 23, 2002·The Journal of Biological Chemistry·Xiaoying YinEdward V Prochownik
Jan 1, 2019·Uirusu·Motoko Ikeda
May 17, 2000·The Journal of General Virology·D K Bideshi, B A Federici

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