Sequential Action of MalE and Maltose Allows Coupling ATP Hydrolysis to Translocation in the MalFGK2 Transporter.

The Journal of Biological Chemistry
Huan BaoFranck Duong

Abstract

ATP-binding cassette (ABC) transporters have evolved an ATP-dependent alternating-access mechanism to transport substrates across membranes. Despite important progress, especially in their structural analysis, it is still unknown how the substrate stimulates ATP hydrolysis, the hallmark of ABC transporters. In this study, we measure the ATP turnover cycle of MalFGK2 in steady and pre-steady state conditions. We show that (i) the basal ATPase activity of MalFGK2 is very low because the cleavage of ATP is rate-limiting, (ii) the binding of open-state MalE to the transporter induces ATP cleavage but leaves release of Pi limiting, and (iii) the additional presence of maltose stimulates release of Pi, and therefore increases the overall ATP turnover cycle. We conclude that open-state MalE stabilizes MalFGK2 in the outward-facing conformation until maltose triggers return to the inward-facing state for substrate and Pi release. This concerted action explains why ATPase activity of MalFGK2 depends on maltose, and why MalE is essential for transport.

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Citations

Feb 9, 2017·Chemical Reviews·Ilia G Denisov, Stephen G Sligar
May 18, 2020·Bulletin of Mathematical Biology·Rebecca M HillerEric N Cytrynbaum
Nov 10, 2020·Protein Science : a Publication of the Protein Society·Stephen G Sligar, Ilia G Denisov
Sep 29, 2019·Research in Microbiology·Rebecca MächtelCédric Orelle
Nov 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Denis LacabanneAnja Böckmann
May 21, 2021·Scientific Reports·Bárbara AbreuCláudio M Soares
Oct 6, 2021·Bioscience, Biotechnology, and Biochemistry·Kenji OkumuraWataru Hashimoto

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