PMID: 8970581Nov 1, 1996Paper

Sequential activation and production of matrix metalloproteinase-2 during breast cancer progression

Clinical & Experimental Metastasis
K S LeeH C Chung

Abstract

The proteolytic processes are thought to be the critical point in tumor invasion and metastasis, mainly by matrix metalloproteinases (MMPs) and serine proteases. We measured the activity of MMP-2 from 28 normal, 12 benign and 126 breast cancer tissues using gelatin zymography. Inactive MMP-2 (72 kD) was expressed in 53.6% of the normal and 66.6% of the cancer tissues, respectively (P = 0.77), while active MMP-2 (62 kD) was expressed in 28.6% and 73.0%, respectively (P = 0.003). The enzymatic activity of active MMP-2 (62 kD) measured in the gel band area was 4.0 +/- 7.2 mm2 in normal breasts, 7.7 +/- 9.8 mm2 in benign breast diseases, 9.5 +/- 8.5 mm2 in ductal carcinoma in situ (DCIS), and 12.0 +/- 13.7 mm2 in invasive cancers. The MMP-2 activation ratio (62 kD/62 kD + 72 kD) was 0.12 +/- 0.18 in normal tissues, 0.10 +/- 0.20 in benign diseases, 0.61 +/- 0.22 in DCIS, and 0.50 +/- 0.28 in invasive cancers. In conclusion, MMP-2 activation was the main cause of the increased 62 kD MMP-2 activity during the early phase of breast cancer, while production of MMP-2 supplemented the increased 62 kD activity in the late phase. We suggest, therefore, that these differential expressions of MMP-2 activation and production during the differ...Continue Reading

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Citations

Dec 30, 2008·Clinical & Experimental Metastasis·Vanity McMurtryAna M Tari
Nov 20, 1998·Breast Cancer Research and Treatment·C BenaudE W Thompson
Jun 17, 2004·Journal of the National Cancer Institute·Gregory D Leonard, Sandra M Swain
Jun 6, 2000·The British Journal of Ophthalmology·C S SethiN H Chong
Jul 31, 2002·Journal of Clinical Pathology·A Rice, C M Quinn
Jun 17, 2006·Breast Cancer Research : BCR·Bernard TêtuDominique Trudel
Mar 26, 2016·Journal of Carcinogenesis·Jessica M Castañeda-Gill, Jamboor K Vishwanatha
May 20, 1998·Cancer Investigation·C J DimitroffR J Bernacki
Nov 22, 2008·The Veterinary Journal·Abigail L ClutterbuckAli Mobasheri
Mar 30, 2000·International Journal of Cancer. Journal International Du Cancer·R HanemaaijerM J Duffy
Oct 2, 2003·British Journal of Cancer·A Talvensaari-MattilaT Turpeenniemi-Hujanen
Dec 21, 2004·Breast Cancer Research and Treatment·Fabienne GrieuBarry Iacopetta

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