PMID: 6161369Nov 1, 1980Paper

Sequential addition of platelet factor and plasma to BALB/c 3T3 fibroblast cultures stimulates somatomedin-C binding early in cell cycle

Proceedings of the National Academy of Sciences of the United States of America
D R ClemmonsW J Pledger

Abstract

Recent studies have shown that confluent cultured fibroblasts must be rendered competent by a factor contained in platelets before they can respond to plasma components and progress from G0 to S phase of the cell cycle. Somatomedin-C is one of the components of platelet-poor plasma necessary for cell cycle progression, but other factors present in somatomedin-C-deficient plasma are also required. Because both competence and progression factors contained in somatomedin-C-deficient serum modulate somatomedin-C action in fibroblasts, this study was undertaken to examine their possible influence on the binding of 125I-labeled somatomedin-C. Exposure of BALB/c 3T3 fibroblasts to high concentrations of platelet-derived growth factor alone prevented the time-dependent decrease in somatomedin-C binding which occurs in serum-free medium. Lower concentrations of the growth factor or 5% (vol/vol) platelet-poor plasma reduced but did not abolish this decrease. In contrast, sequential addition of platelet-derived growth factor followed by platelet-poor plasma resulted in a 75% increase in 125I-labeled somatomedin-C binding over basal levels during the first 2 hr. Binding increased by 125% when somatomedin-C-deficient platelet-poor plasma wa...Continue Reading

References

May 1, 1977·The Journal of Clinical Endocrinology and Metabolism·M M RechlerL Fryklund
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A VogelR Ross
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H N AntoniadesC D Stiles
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J B BakerD D Cunningham
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Das, C F Fox
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pastan
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H N Antoniades, C D Scher
Mar 1, 1979·The Journal of Clinical Endocrinology and Metabolism·A J D'ErcoleA Plet
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J R GavinD N Buell
Mar 15, 1974·Biochemical and Biophysical Research Communications·K MegyesiD M Neville

❮ Previous
Next ❯

Citations

Jan 1, 1987·Cancer Metastasis Reviews·J F WhitfieldP R Walker
Nov 28, 1988·The American Journal of Medicine·R S BarA Sandra
Aug 1, 1983·Molecular and Cellular Endocrinology·E J O'Keefe, W J Pledger
Mar 1, 1985·Molecular and Cellular Endocrinology·C H HeldinB Westermark
Jul 28, 1989·Biochimica Et Biophysica Acta·M Hannink, D J Donoghue
Aug 31, 1992·Biochemical and Biophysical Research Communications·G HaselbacherR E Humbel
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W J PledgerC D Scher
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C Smith, C D Stiles
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A J OwenH N Antoniades
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W WhartonE J O'Keefe
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G L KingC H Heldin
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R G ElginD R Clemmons
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A StellerZ Zou
Dec 31, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Yung-Ming ChangChih-Yang Huang
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yung-Ming ChangChih-Yang Huang
Aug 1, 1996·Alcoholism, Clinical and Experimental Research·M ResnicoffR Rubin
Nov 1, 1985·Journal of the American Academy of Dermatology·R A Clark
Apr 1, 1989·Experimental Cell Research·W E MercerM T Shields
Dec 31, 1987·Biochemical and Biophysical Research Communications·T LinJ F Haskell
Oct 29, 1987·Biochemical and Biophysical Research Communications·R S BarA Cox
Mar 1, 1984·Clinics in Endocrinology and Metabolism
Feb 1, 1982·Experimental Cell Research·M D Bright, E V Gaffney
Mar 1, 1984·Experimental Cell Research·R Baserga
Sep 1, 1989·Journal of Cellular Physiology·E CanalisM Centrella
Mar 12, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R RossE W Raines
Jan 1, 1985·Journal of Cellular Biochemistry·M A HarringtonW J Pledger
Oct 21, 1986·Biochemistry·J M RoweH G Friesen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
W J PledgerC D Scher
Proceedings of the National Academy of Sciences of the United States of America
C D StilesW J Pledger
The Journal of Clinical Endocrinology and Metabolism
D Le Roith, A A Butler
Proceedings of the National Academy of Sciences of the United States of America
A VogelR Ross
© 2021 Meta ULC. All rights reserved