Sequential Collision- and Ozone-Induced Dissociation Enables Assignment of Relative Acyl Chain Position in Triacylglycerols

Analytical Chemistry
David L MarshallStephen J Blanksby

Abstract

Unambiguous identification of isomeric lipids by mass spectrometry represents a significant analytical challenge in contemporary lipidomics. Herein, the combination of collision-induced dissociation (CID) with ozone-induced dissociation (OzID) on an ion-trap mass spectrometer is applied to the identification of triacylglycerol (TG) isomers that vary only by the substitution pattern of fatty acyl (FA) chains esterified to the glycerol backbone. Isolated product ions attributed to loss of a single FA arising from CID of [TG + Na](+) ions react rapidly with ozone within the ion trap. The resulting CID/OzID spectra exhibit abundant ions that unequivocally reveal the relative position of FAs along the backbone. Isomeric TGs containing two or three different FA substituents are readily differentiated by diagnostic ions present in their CID/OzID spectra. Compatibility of this method with chromatographic separations enables the characterization of unusual TGs containing multiple short-chain FAs present in Drosophila.

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Citations

Sep 22, 2016·Analytical Biochemistry·Sarah E HancockTodd W Mitchell
Aug 16, 2016·Rapid Communications in Mass Spectrometry : RCM·David L MarshallStephen J Blanksby
Jan 3, 2019·Angewandte Chemie·Tiffany Porta SiegelShane R Ellis
Jun 22, 2019·Journal of the American Society for Mass Spectrometry·David L MarshallStephen J Blanksby
Jan 1, 2019·Mass Spectrometry·Britt S R ClaesRon M A Heeren
Feb 11, 2021·Cell Reports·Reuben S E YoungStephen J Blanksby
May 29, 2021·Analytical Chemistry·Dong Hao WangJ Thomas Brenna
May 4, 2017·Journal of the American Society for Mass Spectrometry·Andrew P BowmanAlexandre A Shvartsburg
Aug 24, 2017·Journal of the American Society for Mass Spectrometry·Rodell C BarrientosQibin Zhang
Jun 19, 2021·Analytical and Bioanalytical Chemistry·Sven Heiles
Sep 7, 2019·Analytical Chemistry·Luis A MaciasJennifer S Brodbelt
Sep 4, 2020·Journal of the American Society for Mass Spectrometry·J Stuart GrossertLouis Ramaley
Sep 3, 2021·Journal of the American Chemical Society·Carla KirschbaumKevin Pagel

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