PMID: 4729503Aug 1, 1973Paper

Sequential degranulation of the two types of polymorphonuclear leukocyte granules during phagocytosis of microorganisms

The Journal of Cell Biology
D F Bainton

Abstract

The sequential discharge of neutrophilic polymorphonuclear leukocyte (PMN) granules-azurophils and specifics-was investigated by electron microscopy and cytochemistry. Thus the enzyme content of PMN phagocytic vacuoles was determined at brief intervals after phagocytosis of bacteria, utilizing peroxidase as a marker enzyme for azurophil granules, and alkaline phosphatase for specifics. At 30 s, approximately half the phagocytic vacuoles were reactive for alkaline phosphatase, whereas none contained peroxidase. Peroxidase-containing vacuoles were rarely seen at 1 min, but by 3 min, vacuoles containing both enzymes were consistently present. Alkaline phosphatase was found in both small and large vacuoles, whereas peroxidase was visible only in large ones. By 10 min, very big phagocytic vacuoles containing considerable amounts of reaction product for both enzymes were evident. These observations indicate that the two types of PMN granules discharge in a sequential manner, specific granules fusing with the vacuole before azurophils. In an earlier paper, we reported that the pH of phagocytic vacuoles drops to 6.5 within 3 min and to approximately 4 within 7-15 min. Substances known to be present in specific granules (alkaline phosph...Continue Reading

References

Aug 1, 1971·The Journal of Clinical Investigation·T P StosselM Vaughan
Jun 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B K WetzelR G Horn
Jun 2, 1971·Nature: New Biology·G WeissmannR B Zurier
Jan 1, 1972·Annual Review of Medicine·A Janoff
Jan 1, 1971·Annual Review of Medicine·S J Klebanoff
Oct 14, 1969·Annals of the New York Academy of Sciences·M J SchlesingerS Schlesinger
Jan 1, 1966·Annual Review of Physiology·C De Duve, R Wattiaux
Mar 1, 1970·The Journal of Experimental Medicine·M BaggioliniJ F Heremans
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
May 1, 1967·The Journal of Clinical Investigation·S E Malawista, P T Bodel
Dec 1, 1960·The Journal of Experimental Medicine·Z A COHN, J G HIRSCH
Dec 1, 1960·The Journal of Experimental Medicine·J G Hirsch, Z A COHN
Oct 1, 1964·The Journal of Experimental Medicine·D ZUCKER-FRANKLIN, J G Hirsch

Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·E SuzakiS Terakawa
Dec 1, 1987·Biulleten' eksperimental'noĭ biologii i meditsiny·L A MamedovIu R Khrust
Dec 1, 1982·Biulleten' eksperimental'noĭ biologii i meditsiny·O Ia KaufmanV S Paukov
Jul 1, 1979·Inflammation·J D Hunt, P A Ward
Jan 1, 1979·Infection·B ZakhirehR K Root
Jun 18, 1985·Klinische Wochenschrift·M Rister, U Nett
Jan 1, 1978·Agents and Actions·M BaggioliniM E Feigenson
Jan 1, 1979·Folia Microbiologica·M Ferencík, J Stefanovic
Nov 1, 1974·Developmental Biology·R S Decker
Oct 1, 1985·Veterinary Immunology and Immunopathology·N Craven, M R Williams
Jan 1, 1986·International Journal of Immunopharmacology·J GletteC O Solberg
Feb 28, 1974·The New England Journal of Medicine·R G StraussV A Fulginiti
Jan 1, 1975·The Journal of Cell Biology·R T BriggsM J Karnovsky
Sep 1, 1976·The Journal of Cell Biology·R A ClarkS J Klebanoff
Jan 1, 1980·The Journal of Cell Biology·J M ClarkH M Kagan
Sep 1, 1983·The Journal of Cell Biology·P L McNeilD L Taylor
Jan 1, 1978·The Journal of Experimental Medicine·G E HatchD B Menzel
Jan 1, 1975·British Journal of Haematology·D F Bainton
Sep 1, 1976·British Journal of Haematology·H El-Maalem, J Fletcher
Jun 1, 1979·European Journal of Clinical Investigation·M TorresM A Gougerot
Jan 1, 1988·Veterinary Clinical Pathology·J M ValinotiN C Jain
Jul 1, 1978·Journal of Clinical Pathology·J T WozniakJ Stuart
Mar 18, 2005·Annual Review of Immunology·Anthony W Segal
Nov 1, 1975·The Journal of Clinical Investigation·H Odeberg, I Olsson
Feb 1, 1978·The Journal of Clinical Investigation·R D DiamondW Jao
May 1, 1981·The Journal of Clinical Investigation·A A VoetmanD Roos
Sep 1, 1982·The Journal of Clinical Investigation·B DewaldM Baggiolini

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.