Sequential development of histologic lesions and their relationship with bacterial isolation, fecal shedding, and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. paratuberculosis

Veterinary Pathology
N P KuradeN S Parihar

Abstract

Understanding pathogenesis during progressive stages of infection by Mycobacterium avium subsp. paratuberculosis (MAP) and finding suitable methods for its diagnosis are key to the control of Johne's disease in animals. Paratuberculosis was experimentally produced in 20 crossbred lambs by oral administration of MAP to study the sequential development of lesions between 10 and 330 days postinfection and to assess commonly used diagnostic methods such as bacterial culture, lymphocyte stimulation test (LST), and enzyme-linked immunosorbent assay (ELISA) during progressive stages of infection. Histologic lesions were classified into four grades from grade 1 (least severe) to grade 4 (most severe) on the basis of location of granulomatous lesions in different regions and layers of intestines, their association with intestinal lymphoid tissues, pattern and distribution of lesions, types of cellular infiltration, and presence of acid-fast bacilli. It is evident that infection first establishes in lymphoid tissues of the small intestine, possibly at multiple sites, producing segmental lesions and from there spreads to lamina propria and local lymph nodes. Wide variability in the histologic lesions in relation to postinfection periods a...Continue Reading

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Citations

Oct 10, 2009·Tropical Animal Health and Production·Farhan Anwar KhanIjaz Ahmad
Mar 4, 2011·Research in Veterinary Science·Elisa MaioChristian Gortázar
Apr 12, 2008·Veterinary Immunology and Immunopathology·Deborah L TaylorRichard J Whittington
Mar 27, 2015·Risk Analysis : an Official Publication of the Society for Risk Analysis·Kirk J Breuninger, Mark H Weir
Feb 6, 2007·Veterinary Immunology and Immunopathology·A J NedrowM L Thonney
Mar 17, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Douglas J BeggRichard J Whittington
Jan 10, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Shannon A MartinsonShawn L B McKenna
Nov 11, 2017·BMC Veterinary Research·R J WhittingtonK M Plain

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
density gradient centrifugation

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