Sequential expression and differential function of multiple enamel proteins during fetal, neonatal, and early postnatal stages of mouse molar organogenesis

Differentiation; Research in Biological Diversity
H C SlavkinM L Snead

Abstract

We have established the time and position of expression for multiple enamel proteins during the development of the mouse molar tooth organ. Using high-resolution two-dimensional gel electrophoresis coupled with immunoblotting and immunocytochemistry, a 46-kDa enamel protein (pI, 5.5) was detected during late cap stage (18-days gestation, E18d) within differentiation-zone-II inner enamel epithelia associated with an intact basal lamina. At E19d a second enamel polypeptide of 72 kDa (pI, 5.8) was identified at the time and position of initial biomineralization in differentiation zone V. At 20 days, differentiation-zone-VI ameloblasts without basal lamina (late bell stage) expressed 46- and 72-kDa enamel proteins and, in addition, expressed a relatively more basic 26-kDa enamel protein (pI, 6.5-6.7); detected after initial formation of calcium hydroxyapatite crystals. Antibodies raised against chemically synthesized enamel peptides cross-reacted with both the 72-kDa and 26-kDa polypeptides, but did not cross-react with the 46-kDa enamel polypeptide. The sequential expression of multiple enamel proteins suggests several functions: (a) the anionic enamel proteins may provide an instructive template for calcium hydroxyapatite crystal...Continue Reading

References

Mar 1, 1979·Journal of Dental Research·C E Smith
Mar 1, 1979·Journal of Dental Research·R W Fearnhead
Mar 1, 1979·Journal of Dental Research·J E Eastoe
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Jan 1, 1987·Journal of Dental Research·M Zeichner-DavidZ Pavlova
May 1, 1988·The Biochemical Journal·M Zeichner-DavidH C Slavkin
Mar 1, 1987·The Journal of Cell Biology·D R KeeneR E Burgeson
Jun 28, 1985·Biochemical and Biophysical Research Communications·M L SneadH C Slavkin
Nov 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A NanciH C Slavkin
Jan 1, 1974·Oral Sciences Reviews·H C Slavkin
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jun 1, 1968·The Anatomical Record·H Warshawsky
Jun 1, 1971·Journal of Ultrastructure Research·E Kallenbach
Jan 1, 1970·Journal of Ultrastructure Research·E J Reith
Dec 2, 1966·Science·P T LevineJ W Hein
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M L SneadH C Slavkin
Jan 1, 1983·Differentiation; Research in Biological Diversity·M Zeichner-DavidH C Slavkin
Jan 1, 1983·Differentiation; Research in Biological Diversity·G GreenbergH C Slavkin
Jun 15, 1984·Biochemical and Biophysical Research Communications·T TakagiS Sasaki
Jul 1, 1984·Developmental Biology·M L SneadH C Slavkin
Jan 1, 1982·Differentiation; Research in Biological Diversity·H C SlavkinL S Honig
Aug 1, 1982·Journal of Dental Research·A B BelcourtJ D Termine
Jan 1, 1981·Differentiation; Research in Biological Diversity·I Thesleff, K Hurmerinta
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Oct 1, 1981·Bioscience Reports·A G FinchamW C Cothran
Sep 1, 1957·The American Journal of Anatomy·S A COHN

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Citations

Jun 1, 1996·The Anatomical Record·H C Slavkin, T Diekwisch
Jul 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C D FongL Hammarström
Apr 1, 1994·Calcified Tissue International·J P SimmerA G Fincham
Oct 1, 1994·Calcified Tissue International·J P SimmerA G Fincham
Nov 1, 1992·Calcified Tissue International·A KukitaK Kurisu
Dec 1, 1989·Calcified Tissue International·R I CouwenhovenM L Snead
Apr 25, 1989·Biochimica Et Biophysica Acta·H C SlavkinM Zeichner-David
Sep 22, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·M L PaineM L Snead
Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·F BleicherH Magloire
Mar 1, 1992·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·T SakuH Shimokawa
Jul 1, 1992·The Anatomical Record·A NanciC E Smith
May 1, 1991·The Anatomical Record·M D McKeeE C Moreno
Jan 1, 1989·Connective Tissue Research·M Zeichner-DavidH Slavkin
Jan 1, 1989·Connective Tissue Research·C E Smith, A Nanci
Nov 4, 2000·Connective Tissue Research·M J Hubbard
Jan 1, 1989·Connective Tissue Research·A L BronckersJ H Wöltgens
Jan 1, 1995·Connective Tissue Research·M Lagerström-Fermér, U Landegren
Jan 1, 1995·Connective Tissue Research·A G Fincham, J Moradian-Oldak
Jan 1, 1995·Connective Tissue Research·D DeutschA Palmon
Jan 27, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Khalid Al-HezaimiIlan Rotstein
Jul 31, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Zhan HuangMalcolm L Snead
Jan 1, 1991·Archives of Oral Biology·P FargeH Magloire
Jan 1, 1990·Archives of Oral Biology·R C Herold, J Rosenbloom
Jun 1, 1989·The Anatomical Record·A NanciC E Smith
Jun 1, 1989·The Anatomical Record·D Deutsch
Dec 1, 1992·Oral Surgery, Oral Medicine, and Oral Pathology·R P GollardM L Snead
Aug 14, 2015·Clinical Oral Investigations·Yulan WangRichard J Miron
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R Maas, M Bei

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