Sequential expression of B lymphocyte surface antigens in vitro

European Journal of Immunology
J Abbott, K Ngiam

Abstract

Serological techniques were used to examine the process of sequential surface antigen expression on differentiating B cells in vitro. A 4-day culture system is described in which bone marrow lymphocytes from neonatal mice acquire in sequence the ability to express Lyb-2, IgM, Ia and IgD in response to a 3-h induction with E. coli lipopoly-saccharide (LPS). Lyb-2 can be induced on day 1, IgM can be induced after 24 h, Ia after 48 h and IgD only after 96 h in culture. This sequence mimics the order of appearance of B cell surface antigens during ontogeny. When DNA synthesis is blocked from 0.24 h with hydroxyurea (HU), all surface antigens can be induced simultaneously by LPS. Immunoselection of one antigen-bearing population results in the loss of cells bearing other B cell antigens indicating that the surface antigens are induced on the same cells. When both HU and LPS were added to the cultures from the start, IgM appears after 11-14 h, Ia afer 14-15 h and IgD only after 19 h. Induction of antigen was demonstrated by he cytotoxicity assay, quantitative absorption and the protein A sheep red blood cell rosetting assaying. The results obtained show that there is a population of surface IgM-negative precursor B cells in young bon...Continue Reading

References

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Citations

Jan 1, 1984·Developmental and Comparative Immunology·K S Landreth, P W Kincade
Jan 1, 1982·Annals of the New York Academy of Sciences·P W KincadeG Lee

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