Sequential expression of bacterial virulence and plant defense genes during infection of tomato with Clavibacter michiganensis subsp. michiganensis

Phytopathology
Laura ChalupowiczShulamit Manulis-Sasson

Abstract

The molecular interactions between Clavibacter michiganensis subsp. michiganensis and tomato plant were studied by following the expression of bacterial virulence and host-defense genes during early stages of infection. The C. michiganensis subsp. michiganensis genes included the plasmid-borne cellulase (celA) and the serine protease (pat-1), and the serine proteases chpC and ppaA, residing on the chp/tomA pathogenicity island (PAI). Gene expression was measured following tomato inoculation with Cmm382 (wild type), Cmm100 (lacking the plasmids pCM1 and pCM2), and Cmm27 (lacking the PAI). Transcriptional analysis revealed that celA and pat-1 were significantly induced in Cmm382 at initial 12 to 72 h, whereas chpC and ppaA were highly expressed only 96 h after inoculation. Interdependence between the expression of chromosomal and of plasmid-located genes was revealed: expression of celA and pat-1 was substantially reduced in the absence of the chp/tomA PAI, whereas chpC and ppaA expressions were reduced in the absence of the virulence plasmids. Transcription of chromosomal genes involved in cell wall degradation (i.e., pelA1, celB, xysA, and xysB), was also induced at early stages of infection. Expression of the host-defense gene...Continue Reading

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Citations

Aug 5, 2014·Annual Review of Phytopathology·Isaac Barash
Mar 29, 2011·Annual Review of Phytopathology·Rudolf Eichenlaub, Karl-Heinz Gartemann
Sep 3, 2013·Journal of Virological Methods·Przemysław WieczorekAleksandra Obrępalska-Stęplowska
Nov 11, 2016·Plant Signaling & Behavior·Christian Danve M CastroverdeJane Robb
Feb 4, 2019·World Journal of Microbiology & Biotechnology·Guangzheng SunQing Ma
Oct 28, 2020·Plant Disease·F Christopher Peritore-GalveChristine D Smart

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