PMID: 18724692Aug 30, 2008Paper

Sequential expression of Wnt/beta-catenin signal pathway related genes and adipocyte transcription factors during porcine adipose tissue development

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Xiao LuoGong-She Yang

Abstract

To evaluate the influence of Wnt/beta-catenin signal pathway on the porcine adipose tissue development and explore the mechanism, we detected the mRNA expression of Wnt/beta-catenin signal pathway related genes: beta-catenin, GSK3beta, Fzl and adipogenic transcription factors: PPARy, C/EBPalpha and early differentiation marker gene LPL with semi-quantitative (SQ) RT-PCR method. Immunohistochemical method (IHC) was applied to qualitatively measure the sequential expression of beta-catenin protein. The results of SQ RT-PCR showed that beta-catenin highly expressed at the first day after birth, then decreased to a low plateau after 60 days, the expression of GSK3beta and Fzl also decreased with the development process of the porcine adipose tissue development. However, the sequential expression of PPARgamma, C/EBPalpha, LPL appeared to be an opposite manner and kept at a high level after 60 days. The result of IHC showed that the expression of beta-catenin protein was strong in nucleus and cytoplasm at the first day after birth, then tended to decline with the process of adipose tissue development and could be only found in cytoplasm after 30-day old. These results suggest that beta-catenin plays an important role in the undiffere...Continue Reading

Citations

Oct 19, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fei YuJian-Jing Lin
Oct 28, 2010·American Journal of Medical Genetics. Part a·Luitgard M Graul-NeumannEberhard Passarge
Feb 12, 2011·Journal of Cellular Biochemistry·Guoxi LiGongshe Yang

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